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Negative and positive selection of antigen-specific cytotoxic T lymphocytes affected by the α3 domain of MHC I molecules

Aldrich, Carta J. and Hammer, Robert E. and Jones-Youngblood, Sharon and Koszinowski, Ulrich and Hood, Lee and Stroynowski, Iwona and Forman, James (1991) Negative and positive selection of antigen-specific cytotoxic T lymphocytes affected by the α3 domain of MHC I molecules. Nature, 352 (6337). pp. 718-721. ISSN 0028-0836. https://resolver.caltech.edu/CaltechAUTHORS:20150601-142904818

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Abstract

THE α1 and α2 domains of major histocompatibility complex (MHC) class I molecules function in the binding and presentation of foreign peptides to the T-cell antigen receptor and control both negative and positive selection of the T-cell repertoire. Although the α3 domain of class I is not involved in peptide binding, it does interact with the T-cell accessory molecule, CDS. CDS is important in the selection of T cells as anti-CDS antibody injected into perinatal mice interfers with this process. We previously used a hybrid class I molecule with the α1/α2 domains from L^d and the α3 domain from Q7^b and showed that this molecule binds an L^d-restricted peptide but does not interact with CD8-dependent cytotoxic T lymphocytes. Expression of this molecule in transgenic mice fails to negatively select a subpopulation of anti-L^d cytotoxic T lymphocytes. In addition, positive selection of virus-specific L^d-restricted cytotoxic T lymphocytes does not occur. We conclude that besides the α1/α2 domains of class I, the α3 domain plays an important part in both positive and negative selection of antigen-specific cells.


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http://dx.doi.org/10.1038/352718a0DOIArticle
http://www.nature.com/nature/journal/v352/n6337/abs/352718a0.htmlPublisherArticle
Additional Information:© 1991 Nature Publishing Group. Received 26 March; Accepted 4 July 1991. This work was supported by an NIH grant (J.F.). We thank M. Zuniga for the L^d gene. G. D. Senn Ill for technical assistance, and B. Washington for secretarial help.
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Issue or Number:6337
Record Number:CaltechAUTHORS:20150601-142904818
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150601-142904818
Official Citation:Aldrich, C. J., Hammer, R. E., Jones-Youngblood, S., Koszinowski, U., Hood, L., Stroynowski, I., & Forman, J. (1991). Negative and positive selection of antigen-specific cytotoxic T lymphocytes affected by the [alpha]3 domain of MHC I molecules. [10.1038/352718a0]. Nature, 352(6337), 718-721.
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ID Code:57924
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:01 Jun 2015 22:40
Last Modified:03 Oct 2019 08:30

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