Circular Deoxyribonucleic Acid from Shigella dysenteriae Y6R

Rush, Mark G. and Gordon, C. N. and Warner, Robert C. (1969) Circular Deoxyribonucleic Acid from Shigella dysenteriae Y6R. Journal of Bacteriology, 100 (2). pp. 803-808. ISSN 0021-9193. PMCID PMC250161. https://resolver.caltech.edu/CaltechAUTHORS:RUSjbact69

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Abstract

Circular deoxyribonucleic acid was isolated from Shigella dysenteriae Y6R and was found to consist of six species having molecular weights of 10^6, 1.3 x 10^6, 2.6 x 10^6, 3.8 x 10^6, 20 x 10^6, and 24 x 10^6 daltons. These size classes were partially resolved by sucrose density gradient centrifugation. The minicircles (10^6 and 1.3 x 10^6) were found to have a buoyant density in CsCl of 1.710 g/ml. The 3.8 x 10^6 dalton class had a density of 1.707 g/ml. The two largest species had a density of 1.702 g/ml. Two other strains, S. sonnei II and S. dysenteriae 60, also contained circular deoxyribonucleic acid.

Item Type:

Article

Related URLs:

URL	URL Type	Description
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC250161/	PubMed Central	Article
http://jb.asm.org/cgi/content/abstract/100/2/803	Other	UNSPECIFIED
http://jb.asm.org/cgi/content/abstract/100/2/803	Other	UNSPECIFIED

Additional Information:

© 1969 American Society for Microbiology. Received for publication 18 August 1969 This research was supported by Public Health Service grant GM 06967 from the National Institute of General Medical Sciences, by a grant for postdoctoral research from Merck and Co. (M.G.R.), by Public Health Service career award K3 GM 14899 (R.C.W.), and by a predoctoral fellowship from Public Health Service training grant 5 Ti GM 1234 (C.N.G.) The expert assistance of Dymetria Rush is gratefully acknowledged. ADDENDUM IN PROOF: Circular DNA has also been isolated from Shigella by H.S. Jansz, J. Zandberg, J.H. vander Pol, and E.F.J. van Bruggen (Eur. J. Biochem. 9:156, 1969).

Funders:

Funding Agency	Grant Number
NIH	GM 06967
Merck and Company	UNSPECIFIED
NIH	K3 GM 14899
NIH Predoctoral Fellowship	5 Ti GM 1234

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PubMed Central ID:

PMC250161

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CaltechAUTHORS:RUSjbact69

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https://resolver.caltech.edu/CaltechAUTHORS:RUSjbact69

Official Citation:

Circular Deoxyribonucleic Acid from Shigella dysenteriae Y6R Mark G. Rush, C. N. Gordon, Robert C. Warner Journal of Bacteriology Nov 1969, 100 (2) 803-808

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5795

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CaltechAUTHORS

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Deposited On:

02 Nov 2006

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02 Oct 2019 23:26

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