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Separable bilayer microfiltration device for viable label-free enrichment of circulating tumor cells

Zhou, Ming-Da and Hao, Sijie and Williams, Anthony J. and Lv, Bo and Zhu, Jiyue and Cote, Richard J. and Datar, Ram H. and Tai, Yu-Chong and Zheng, Siyang (2014) Separable bilayer microfiltration device for viable label-free enrichment of circulating tumor cells. Cancer Research, 74 (19 Suppleme). Art. No. LB-193. ISSN 0008-5472. http://resolver.caltech.edu/CaltechAUTHORS:20150604-145855669

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Abstract

We designed a new separable bilayer (SB) microfiltration device for the viable enrichment of circulating tumor cells (CTCs) independent of antigen expression. Bilayer pore structures at the micro scale decrease cell damage to preserve viability, while maintaining throughput to allow rapid enrichment. The large pore size (40 um) of the upper layer facilitates the CTCs proliferation on chip. The separability enables the efficient release of the CTCs after successful on chip culture of captured CTCs. We characterized the device performances including capture efficiency, enrichment against leukocytes, viability and proliferability. The SB device can enrich tumor cells with 80% capture efficiency, higher than 10^3 enrichment, and better than 70% viability from 1 mL whole blood samples on a 1 cm^2 device. Multiple cell lines were shown to be able to proliferate after captured on SB device. Furthermore, using CTCs derived from an in vivo model system, cell cultures were established by releasing the captured CTCs from the chip. The cultured CTCs and their corresponding parental cell lines were injected into mice. Tumors were established with no discernable differences in volume and rate of growth, demonstrating similar tumorigenicity of viable CTCs recovered by the SB device. In a feasibility study, we successfully detected CTCs from 1 mL of clinical whole blood sample enriched via SB device.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1158/1538-7445.AM2014-LB-193 DOIArticle
http://cancerres.aacrjournals.org/content/74/19_Supplement/LB-193PublisherArticle
ORCID:
AuthorORCID
Tai, Yu-Chong0000-0001-8529-106X
Additional Information:©2014 American Association for Cancer Research.
Record Number:CaltechAUTHORS:20150604-145855669
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150604-145855669
Official Citation:Ming-Da Zhou, Sijie Hao, Anthony J. Williams, Bo Lv, Jiyue Zhu, Richard J. Cote, Ram H. Datar, Yu-Chong Tai, Siyang Zheng. Separable bilayer microfiltration device for viable label-free enrichment of circulating tumor cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr LB-193. doi:10.1158/1538-7445.AM2014-LB-193
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:58017
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:05 Jun 2015 03:39
Last Modified:06 Oct 2017 00:35

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