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Applications of Natural-Abundance Nitrogen-15 Nuclear Magnetic Resonance to Large Biochemically Important Molecules

Gust, Devens and Moon, Richard B. and Roberts, John D. (1975) Applications of Natural-Abundance Nitrogen-15 Nuclear Magnetic Resonance to Large Biochemically Important Molecules. Proceedings of the National Academy of Sciences of the United States of America, 72 (12). pp. 4696-4700. ISSN 0027-8424.

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Natural-abundance nitrogen-15 nuclear magnetic resonance spectroscopy of enzymes and other biopolymers is found to be feasible using newly available instrumentation. The long correlation times of such molecules result in short spin-lattice relaxation times, and these in turn allow rapid signal accumulation. The advantages of short T1 values are sometimes offset, however, by unfavorable nuclear Overhauser effects. The dependence of T1 and nuclear Overhauser effects upon correlation time is discussed, and preliminary nitrogen-15 nuclear magnetic resonance results for several biopolymers, including lysozyme, protamines, pepsin, hemoglobin, vitamin B12, and tRNA, are presented.

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Additional Information:Copyright © 1975 by the National Academy of Sciences Contributed by John D. Roberts, August 4, 1975 This is contribution no. 5150 from the Gates and Crellin Laboratories of Chemistry. These studies were supported by the National Science Foundation, and by the Public Health Service, Research Grant no. GM-11072, from the Division of General Medical Sciences. We are indebted to Dr. Craig Bradley of Bruker Magnetics for originating many of the design elements of the spectrometer and to Dr. Philip Loftus for greatly extending the computer software to permit efficient operation of the instrument for determination of relaxation times and nuclear Overhauser effects.
Subject Keywords:chemical shift; spin-lattice relaxation; nuclear Overhauser effect; biopolymers
Issue or Number:12
Record Number:CaltechAUTHORS:GUSpnas75
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5804
Deposited By: Archive Administrator
Deposited On:02 Nov 2006
Last Modified:02 Oct 2019 23:26

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