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A polycystic kidney-disease gene homologue required for male mating behaviour in C-elegans

Barr, Maureen M. and Sternberg, Paul W. (1999) A polycystic kidney-disease gene homologue required for male mating behaviour in C-elegans. Nature, 401 (6751). pp. 386-389. ISSN 0028-0836. doi:10.1038/43916. https://resolver.caltech.edu/CaltechAUTHORS:20150605-111134546

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Abstract

The stereotyped mating behaviour of the Caenorhabditis elegans male is made up of several substeps: response, backing, turning, vulva location, spicule insertion and sperm transfer. The complexity of this behaviour is reflected in the sexually dimorphic anatomy and nervous system. Behavioural functions have been assigned to most of the male-specific sensory neurons by means of cell ablations; for example, the hook sensory neurons HOA and HOB are specifically required for vulva location. We have investigated how sensory perception of the hermaphrodite by the C. elegans male controls mating behaviours. Here we identify a gene, lov-1 (for location of vulva), that is required for two male sensory behaviours: response and vulva location. lov-1 encodes a putative membrane protein with a mucin-like, serine–threonine-rich amino terminus followed by two blocks of homology to human polycystins, products of the autosomal dominant polycystic kidney-disease loci PKD1 and PKD2 (ref 4). LOV-1 is the closest C. elegans homologue of PKD1. lov-1 is expressed in adult males in sensory neurons of the rays, hook and head, which mediate response, vulva location, and potentially chemotaxis to hermaphrodites, respectively. PKD-2, the C. elegans homologue of PKD2, is localized to the same neurons as LOV-1, suggesting that they function in the same pathway.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/43916 DOIArticle
http://www.nature.com/nature/journal/v401/n6751/full/401386a0.htmlPublisherArticle
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 1999 Macmillan Magazines Ltd. Received 23 April; accepted 16 July 1999. We thank J. Copeland for help isolating lov-1(sy582Δ); L. Jiang, R. Garcia and R. Ballester for discussions and constructive criticisms; members of our lab, S. Myers and M. Snow for experimental suggestions; D. Sherwood and B. Smith for assistance with confocal microscopy; A. Fire for vectors; and R. Herman for osm-6::gfp. The Caenorhabditis Genetics stock Center and Sanger Center provided numerous strains, cosmids and sequencing data. This work was supported by the Howard Hughes Medical Institute, with which P.W.S. is an investigator and M.M.B. is an associate, and by the Seaver Institute.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Seaver InstituteUNSPECIFIED
Issue or Number:6751
DOI:10.1038/43916
Record Number:CaltechAUTHORS:20150605-111134546
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150605-111134546
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:58043
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:05 Jun 2015 18:16
Last Modified:10 Nov 2021 21:58

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