Schuster, Stephan C. and Swanson, Ronald V. and Alex, Lisa A. and Bourret, Robert B. and Simon, Melvin I. (1993) Assembly and function of a quaternary signal transduction complex monitored by surface plasmon resonance. Nature, 365 (6444). pp. 343-347. ISSN 0028-0836. https://resolver.caltech.edu/CaltechAUTHORS:20150609-123819914
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Abstract
We have used surface plasmon resonance biosensor technology to monitor the assembly and dynamics of a signal transduction complex which controls chemotaxis in Escherichia coli A quaternary complex formed which consisted of the response regulator CheY, the histidine protein kinase CheA, a coupling protein CheW and a membrane-bound chemoreceptor Tar. Using various experimental conditions and mutant proteins, we have shown that the complex dissociates under conditions that favour phosphorylation of CheY. Direct physical analysis of interactions among proteins in this signal transduction pathway provides evidence for a previously unrecognized binding interaction between the kinase and its substrate. This interaction may be important for enhancing substrate specificity and preventing 'crosstalk' with other systems. The approach is generally applicable to furthering our understanding of how signalling complexes transduce intracellular messages.
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Additional Information: | © 1993 Nature Publishing Group. Received 23 April; accepted 1 July 1993. This work was supported by a grant from the US Public Health Service to M.I.S. S.C.S. is a recipient of a Max-Planck-Gesellschaft Award Fellowship, R.V.S. is supported by a National Research Service Award Fellowship and L.A.A. is supported by an American Cancer Society Fellowship. | ||||||||||
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Issue or Number: | 6444 | ||||||||||
Record Number: | CaltechAUTHORS:20150609-123819914 | ||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150609-123819914 | ||||||||||
Official Citation: | Assembly and function of a quaternary signal transduction complex monitored by surface plasmon resonance 343 Stephan C. Schuster, Ronald V. Swanson, Lisa A. Alex, Robert B. Bourret & Melvin I. Simon doi:10.1038/365343a0 | ||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
ID Code: | 58127 | ||||||||||
Collection: | CaltechAUTHORS | ||||||||||
Deposited By: | Ruth Sustaita | ||||||||||
Deposited On: | 09 Jun 2015 22:09 | ||||||||||
Last Modified: | 03 Oct 2019 08:32 |
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