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Subunits βy of heterotrimeric G protein activate β2 isoform of phospholipase C

Katz, Arieh and Wu, Dianqing and Simon, Melvin I. (1992) Subunits βy of heterotrimeric G protein activate β2 isoform of phospholipase C. Nature, 360 (6405). pp. 686-689. ISSN 0028-0836. doi:10.1038/360686a0.

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THE activation of heterotrimeric G proteins results in the exchange of GDP bound to the α-subunit for GTP and the subsequent dissociation of a complex of the β- and γ-subunits (Gβγ). The α -subunits of different G proteins interact with a variety of effectors, but less is known about the function of the free Gβγ complex. Gβγ has been implicated in the activation of a cardiac potassium channel, a retinal phospholipase A2 (ref. 9) and a specific receptor kinase, and in vitro reconstitution experiments indicate that the Gβγ complex can act with Gα subunit to modulate the activity of different isoforms of adenylyl cyclase11. Of two phospholipase activities that can be separated in extracts of HL-60 cells, purified Gβγ is found to activate one of them. Here we report that in co-transfection assays Gβγ subunits specifically activate the β2 and not the β1 isoform of phospholipase, which acts on phosphatidylinositol. We use transfection assays to show also that receptor-mediated release of Gβγ from G proteins that are sensitive to pertussis toxin can result in activation of the phospholipase. This effect may be the basis of the pertussis-toxin-sensitive phospholipase C activation seen in some cell systems (reviewed in refs 13 and 14).

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Katz, Arieh0000-0003-3487-7049
Additional Information:© 1992 Nature Publishing Group. Received 12 June; accepted 27 October 1992. A.K. and D.W. contributed equally to this work. We thank S. G. Rhee, J. Knopf. E. Peralta, W. Simonds and R. Perlmutter for cDNA clones and antisera. This work is supported by a US public Health Services grant to M.l.S.
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US Public Health ServiceUNSPECIFIED
Issue or Number:6405
Record Number:CaltechAUTHORS:20150609-132537326
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Official Citation:Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C 686 Arieh Katz, Dianqing Wu & Melvin I. Simon doi:10.1038/360686a0
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:58131
Deposited By: Ruth Sustaita
Deposited On:10 Jun 2015 15:39
Last Modified:10 Nov 2021 21:59

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