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Recent advances in engineering microbial rhodopsins for optogenetics

McIsaac, R. Scott and Bedbrook, Claire N. and Arnold, Frances H. (2015) Recent advances in engineering microbial rhodopsins for optogenetics. Current Opinion in Structural Biology, 33 . pp. 8-15. ISSN 0959-440X. PMCID PMC4641784. doi:10.1016/j.sbi.2015.05.001. https://resolver.caltech.edu/CaltechAUTHORS:20150612-152121122

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Abstract

Protein engineering of microbial rhodopsins has been successful in generating variants with improved properties for applications in optogenetics. Members of this membrane protein family can act as both actuators and sensors of neuronal activity. Chimeragenesis, structure-guided mutagenesis, and directed evolution have proven effective strategies for tuning absorption wavelength, altering ion specificity and increasing fluorescence. These approaches facilitate the development of useful optogenetic tools and, in some cases, have yielded insights into rhodopsin structure–function relationships.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.sbi.2015.05.001DOIArticle
http://www.sciencedirect.com/science/article/pii/S0959440X15000615PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4641784PubMed CentralArticle
ORCID:
AuthorORCID
McIsaac, R. Scott0000-0002-5339-6032
Bedbrook, Claire N.0000-0003-3973-598X
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2015 Elsevier Ltd. This work was funded by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office (to F.H.A.) and 1R21MH103824-01 from the National Institutes of Health (to F.H.A.). R.S.M. acknowledges funding from the Shurl and Kay Curci Foundation and the Life Sciences Research Foundation. C.N.B. acknowledges support from the NIMH of the NIH for the NRSA fellowship under Award Number F31MH102913.
Funders:
Funding AgencyGrant Number
Army Research Office (ARO)W911NF-09-0001
NIH1R21MH103824-01
Shurl and Kay Curci FoundationUNSPECIFIED
Life Sciences Research FoundationUNSPECIFIED
NIH Predoctoral FellowshipF31MH102913
National Institute of Mental Health (NIMH)UNSPECIFIED
PubMed Central ID:PMC4641784
DOI:10.1016/j.sbi.2015.05.001
Record Number:CaltechAUTHORS:20150612-152121122
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150612-152121122
Official Citation:R Scott McIsaac, Claire N Bedbrook, Frances H Arnold, Recent advances in engineering microbial rhodopsins for optogenetics, Current Opinion in Structural Biology, Volume 33, August 2015, Pages 8-15, ISSN 0959-440X, http://dx.doi.org/10.1016/j.sbi.2015.05.001. (http://www.sciencedirect.com/science/article/pii/S0959440X15000615)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:58223
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 Jun 2015 01:03
Last Modified:10 Nov 2021 22:01

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