A Caltech Library Service

A Multiplexed, Two-Electrode Platform for Biosensing Based on DNA-Mediated Charge Transport

Furst, Ariel L. and Hill, Michael G. and Barton, Jacqueline K. (2015) A Multiplexed, Two-Electrode Platform for Biosensing Based on DNA-Mediated Charge Transport. Langmuir, 31 (23). pp. 6554-6562. ISSN 0743-7463. PMCID PMC4587567. doi:10.1021/acs.langmuir.5b00829.

[img] PDF - Accepted Version
See Usage Policy.

[img] PDF (Additional experimental details as well as a CV of the copper precatalyst, impedance spectrometry data of the DNA monolayers, and spacer height optimization data) - Supplemental Material
See Usage Policy.


Use this Persistent URL to link to this item:


We have developed a thin layer, multiplexed biosensing platform that features two working-electrode arrays for detecting small molecules, nucleic acid sequences, and DNA-binding proteins. DNA duplexes are patterned onto the primary electrode array, while a secondary electrode array is used both to initiate DNA monolayer formation and for electrochemical readout via DNA-mediated charge transport (DNA CT) chemistry. Electrochemical reduction of Cu(phendione)_2^(2+) (phendione is 1,10-phenanthroline-5,6-dione) at the secondary electrodes induces covalent attachment via click chemistry of ethynyl-labeled DNA probe duplexes onto the primary electrodes that have been treated with azide-terminated alkylthiols. Electrochemical impedance spectroscopy and cyclic voltammetry confirm that catalyst activation at the secondary electrode is essential to maintain the integrity of the DNA monolayer. Electrochemical readout of DNA CT processes that occur at the primary electrode is accomplished also at the secondary electrode. The two-electrode system enables the platform to function as a collector–generator using either ferrocyanide or ferricyanide as mediators with methylene blue and DNA charge transport. Electrochemical measurements at the secondary electrode eliminate the need for large background corrections. The resulting sensitivity of this platform enables the reliable and simultaneous detection of femtomoles of the transcription factors TATA-binding protein and CopG on a single multiplexed device.

Item Type:Article
Related URLs:
URLURL TypeDescription Information CentralArticle
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2015 American Chemical Society. Received: March 10, 2015; Revised: May 22, 2015; Publication Date (Web): June 4, 2015. We are grateful to the NIH (GM61077) for their financial support of this research. The authors declare no competing financial interest.
Funding AgencyGrant Number
Issue or Number:23
PubMed Central ID:PMC4587567
Record Number:CaltechAUTHORS:20150615-083148726
Persistent URL:
Official Citation:A Multiplexed, Two-Electrode Platform for Biosensing Based on DNA-Mediated Charge Transport Ariel L. Furst, Michael G. Hill, and Jacqueline K. Barton Langmuir 2015 31 (23), 6554-6562 DOI: 10.1021/acs.langmuir.5b00829
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:58231
Deposited By: Tony Diaz
Deposited On:15 Jun 2015 21:32
Last Modified:10 Nov 2021 22:01

Repository Staff Only: item control page