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Quantitative analysis of the GAL4/UAS system in Drosophila oogenesis

Goentoro, Lea A. and Yakoby, Nir and Goodhouse, Joseph and Schüpbach, Trudi and Shvartsman, Stanislav Y. (2006) Quantitative analysis of the GAL4/UAS system in Drosophila oogenesis. Genesis, 44 (2). pp. 66-74. ISSN 1526-954X. doi:10.1002/gene.20184. https://resolver.caltech.edu/CaltechAUTHORS:20150616-090526032

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Abstract

The GAL4/UAS system is extensively used for targeted gene expression in Drosophila, but the strength of the GAL4 drivers and their effects on target genes are rarely quantified. Quantitative information about the strength of the perturbations introduced by the GAL4/UAS system would further expand the usefulness of the GAL4/UAS system in studying gene functions and developmental processes. We have developed an assay to determine the relative level of expression for target genes tagged with green fluorescent protein (GFP). Our assay enables the relative quantitation of fluorescent proteins within specific cell types and developmental time windows in living eggs/embryos, and permits the analysis of samples from a broad expression range. We illustrate the assay using a panel of four GAL4 drivers and three UAS responder lines in Drosophila oogenesis, discuss the issues associated with the interpretation of the quantitative data, and correlate our results with the analysis of the GAL4/UAS system at the transcript level. The imaging-based strategy described here can be used to quantify other GAL4 drivers in Drosophila and other organisms.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/gene.20184DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/gene.20184/abstractPublisherArticle
ORCID:
AuthorORCID
Goentoro, Lea A.0000-0002-3904-0195
Additional Information:© 2006 Wiley-Liss. Received 26 July 2005; Accepted 19 November 2005. The authors thank Horacio Frydman, Eric Wieschaus, Kevin Foster, Elizabeth Gavis, and Joseph Duffy for help and discussion on the fluorescence assay. SYS, LAG, and NY were supported by funds from the National Science Foundation (NSF), NIGMS (National Institute of General Medical Sciences), Searle Scholar Program, Sloan Foundation, and the Burroughs Wellcome Fund (BWF).
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
National Institute of General Medical SciencesUNSPECIFIED
Searle Scholars ProgramUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Burroughs Wellcome FundUNSPECIFIED
Subject Keywords:quantitative fluorescence; Drosophila; GAL4/UAS; GFP; EGFP; EGFR
Issue or Number:2
DOI:10.1002/gene.20184
Record Number:CaltechAUTHORS:20150616-090526032
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150616-090526032
Official Citation:Goentoro, L. A., Yakoby, N., Goodhouse, J., Schüpbach, T. and Shvartsman, S. Y. (2006), Quantitative analysis of the GAL4/UAS system in Drosophila oogenesis. Genesis, 44: 66–74. doi: 10.1002/gene.20184
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:58278
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:16 Jun 2015 18:04
Last Modified:10 Nov 2021 22:02

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