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Prolonged photoresponses in transgenic mouse rods lacking arrestin

Xu, Jun and Dodd, Robert L. and Makino, Clint L. and Simon, Melvin I. and Baylor, Denis A. and Chen, Jeannie (1997) Prolonged photoresponses in transgenic mouse rods lacking arrestin. Nature, 389 (6650). pp. 505-509. ISSN 0028-0836. doi:10.1038/39068.

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Arrestins are soluble cytoplasmic proteins that bind to G-protein-coupled receptors, thus switching off activation of the G protein and terminating the signalling pathway that triggers the cellular response. Although visual arrestin has been shown to quench the catalytic activity of photoexcited, phosphorylated rhodopsin in a reconstituted system, its role in the intact rod cell remains unclear because phosphorylation alone reduces the catalytic activity of rhodopsin. Here we have recorded photocurrents of rods from transgenic mice in which one or both copies of the arrestin gene were disrupted. Photoresponses were unaffected when arrestin expression was halved, indicating that arrestin binding is not rate limiting for recovery of the rod photoresponse, as it is in Drosophila . With arrestin absent, the flash response displayed a rapid partial recovery followed by a prolonged final phase. This behaviour indicates that an arrestin-independent mechanism initiates the quench of rhodopsin's catalytic activity and that arrestin completes the quench. The intensity dependence of the photoresponse in rods lacking arrestin further suggests that, although arrestin is required for normal signal termination, it does not participate directly in light adaptation.

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Additional Information:© 1997 Macmillan Publishers Ltd. Received 3 April; accepted 2 July 1997. This work was supported by grants from the National Eye Institute (D.A.B.), the National Institute on Aging (M.I.S.), the National Institute of General Medical Sciences (R.L.D.), the Ruth and Milton Steinbach Fund (D.A.B., J.C.), The McKnight Endowment Fund for Neuroscience (D.A.B.), Research to Prevent Blindness (J.C., C.L.M.) and Lions’ of Massachusetts (C.L.M.).
Funding AgencyGrant Number
National Eye InstituteUNSPECIFIED
National Institute on AgingUNSPECIFIED
National Institute of General Medical SciencesUNSPECIFIED
Ruth and Milton Steinbach FundUNSPECIFIED
McKnight Endowment Fund for NeuroscienceUNSPECIFIED
Research to Prevent BlindnessUNSPECIFIED
Lions' of MassachusettsUNSPECIFIED
Issue or Number:6650
Record Number:CaltechAUTHORS:20150617-145235626
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Official Citation:Prolonged photoresponses in transgenic mouse rods lacking arrestin p505 Jun Xu, Robert L. Dodd, Clint L. Makino, Melvin I. Simon, Denis A. Baylor and Jeannie Chen doi:10.1038/39068
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:58332
Deposited By: Ruth Sustaita
Deposited On:17 Jun 2015 22:31
Last Modified:10 Nov 2021 22:03

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