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Escherichia coli Disinfection by Electrohydraulic Discharges

Ching, W.-K. and Colussi, A. J. and Sun, H. J. and Nealson, K. H. and Hoffmann, M. R. (2001) Escherichia coli Disinfection by Electrohydraulic Discharges. Environmental Science and Technology, 35 (20). pp. 4139-4144. ISSN 0013-936X. https://resolver.caltech.edu/CaltechAUTHORS:20150623-154757677

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Abstract

We study the survival of single-strain Escherichia coli colonies in aqueous media exposed to 5.5 kV, 90 kA electrohydraulic discharges (EHD). The probability of survival (P_n) of a 4 × 10^7 cfu mL^(-1) E. coli population after n consecutive EHDs follows a logit distribution:  ln(P_n/100 − P_n) = 1.329 − 1.579 ln n with r^2 = 0.993 that corresponds to lethal doses of LD_(50) = 2.2 and LD_(90) = 10.5 EHDs. Considering that the reactor is thoroughly mixed during each discharge and that LD_(50) = 0.9 values are nearly independent of E. coli concentrations in the range of 2 × 10^3 ≤ E. coli/cfu mL^(-1) ≤ 3 × 10^6, we ascribe the nonexponential P_n decay of single-strain E. coli colonies to a shielding phenomenon where inactive cells protect the successively smaller numbers of viable cells in the EHD. The qualitatively similar concentration dependence observed for survival under 254 nm of radiation, in contrast with the lower resistance of denser colonies to 20 kHz power ultrasound and the delayed onset of extracellular β-d-galactosidase activity in bacterial populations already decimated by EHDs, support the view that UV radiation is the dominant disinfection agent generated by electrohydraulic discharges.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/es010643uDOIArticle
http://pubs.acs.org/doi/abs/10.1021/es010643uPublisherArticle
ORCID:
AuthorORCID
Colussi, A. J.0000-0002-3400-4101
Nealson, K. H.0000-0001-5189-3732
Hoffmann, M. R.0000-0002-0432-6564
Additional Information:© 2001 American Chemical Society. Received for review February 14, 2001. Revised manuscript received July 31, 2001. Accepted July 31, 2001. Financial support for this project was provided by the following agencies:  DARPA, ONR, and EPRI.
Funders:
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
Office of Naval Research (ONR)UNSPECIFIED
Electric Power Research Institute (EPRI)UNSPECIFIED
Issue or Number:20
Record Number:CaltechAUTHORS:20150623-154757677
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150623-154757677
Official Citation:Escherichia coli Disinfection by Electrohydraulic Discharges W.-K. Ching, A. J. Colussi, H. J. Sun, K. H. Nealson, and M. R. Hoffmann Environmental Science & Technology 2001 35 (20), 4139-4144 DOI: 10.1021/es010643u
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:58483
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:28 Jul 2015 20:23
Last Modified:03 Oct 2019 08:36

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