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Localized perturbations in CheY structure monitored by NMR identify a CheA binding interface

Swanson, Ronald V. and Lowry, David F. and Matsumura, Philip and McEvoy, Megan M. and Simon, Melvin I. and Dahlquist, Frederick W. (1995) Localized perturbations in CheY structure monitored by NMR identify a CheA binding interface. Nature Structural Biology, 2 (10). pp. 906-910. ISSN 1072-8368. doi:10.1038/nsb1095-906. https://resolver.caltech.edu/CaltechAUTHORS:20150624-142751543

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Abstract

Phosphotransfer between the autophosphorylating histidine kinase CheA and the response regulator CheY represents a crucial step in the bacterial chemotaxis signal transduction pathway. The ^(15)N−^1H correlation spectrum of CheY complexed with an amino-terminal fragment of CheA exhibits specific localized differences in chemical shifts when compared to the spectrum of uncompiexed CheY. When mapped onto the three-dimensional structure of CheY, these changes define a region distinct from the active site. A single amino-acid substitution within this binding region on CheY, alanine to valine at position 103, significantly decreases the affinity of CheY for CheA. The binding face described by these changes partially overlaps a flagellar switch binding surface previously defined by mutagenesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/nsb1095-906DOIArticle
http://www.nature.com/nsmb/journal/v2/n10/abs/nsb1095-906.htmlPublisherArticle
Additional Information:© 1995 Nature Publishing Group. Received 7 July; Accepted 7 August 1995. Supported by U S. Public Health Service Grants (M.I.S), (F.W.D), (P.M.), Cancer Research Fund of the Damon Runyon-Walter Winchell Foundation Fellowship (D.F.L.), National Research Service Award Fellowship (R.V.S.), and a National Research Service Award Pre-doctoral Training Grant from NIH (M.M.M).
Funders:
Funding AgencyGrant Number
U. S. Public Health Service (USPHS)UNSPECIFIED
Damon Runyon-Walter Winchell Cancer FundUNSPECIFIED
National Research Service AwardUNSPECIFIED
NIHUNSPECIFIED
Issue or Number:10
DOI:10.1038/nsb1095-906
Record Number:CaltechAUTHORS:20150624-142751543
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150624-142751543
Official Citation:Swanson, R. V., Lowry, D. F., Matsumura, P., McEvoy, M. M., Simon, M. I., & Dahlquist, F. W. (1995). Localized perturbations in CheY structure monitored by NMR identify a CheA binding interface. [10.1038/nsb1095-906]. Nat Struct Mol Biol, 2(10), 906-910.
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ID Code:58589
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:24 Jun 2015 21:57
Last Modified:10 Nov 2021 22:07

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