CaltechAUTHORS
  A Caltech Library Service

Clones of individual repetitive sequences from sea urchin DNA constructed with synthetic Eco RI sites

Scheller, Richard H. and Thomas, Terry L. and Lee, Amy S. and Klein, William H. and Niles, Walter D. and Britten, Roy J. and Davidson, Eric H. (1977) Clones of individual repetitive sequences from sea urchin DNA constructed with synthetic Eco RI sites. Science, 196 (4286). pp. 197-200. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20150630-123440728

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20150630-123440728

Abstract

Interspersed repetitive sequences were isolated from sea urchin DNA by renaturing to low C_0t followed by treatment with nuclease SI. Synthetic Eco RI sites were ligated onto the repetitive sequence elements, which were then inserted at the Eco RI site of plasmid RSF2124 and cloned. The repetitive sequences can be excised from the plasmid with Eco RI for further study.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.847467DOIArticle
http://www.sciencemag.org/content/196/4286/197PublisherArticle
http://www.jstor.org/stable/1743691JSTORArticle
Additional Information:© 1977 American Association for the Advancement of Science. Received 7 February 1977. T4 polynucleotide kinase, T4 DNA ligase, and Eco RI endonuclease were gifts of P. Green, H. Heyneker, and H. Boyer. Supported by NIH grants HD-05753 and GM-20927 and by NSF grant BMS 75-07359. R.H.S. is supported by an NIH predoctoral training grant; T.L.T. by an NIH postdoctoral fellowship; A.S.L. by fellowship J-289 and W.H.K. by Lievre fellowship J-340, both from the American Cancer Society, California Division.
Funders:
Funding AgencyGrant Number
NIHHD-05753
NIHGM-20927
NSFBMS 75-07359
NIHUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Record Number:CaltechAUTHORS:20150630-123440728
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150630-123440728
Official Citation:Scheller, R., Thomas, T., Lee, A., Klein, W., Niles, W., Britten, R., & Davidson, E. (1977). Clones of individual repetitive sequences from sea urchin DNA constructed with synthetic Eco RI sites. Science, 196(4286), 197-200. doi: 10.1126/science.847467
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:58710
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:01 Jul 2015 15:16
Last Modified:13 Feb 2019 18:06

Repository Staff Only: item control page