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Chemical synthesis of restriction enzyme recognition sites useful for cloning

Scheller, Richard H. and Dickerson, Richard E. and Boyer, Herbert W. and Riggs, Arthur D. and Itakura, Keiichi (1977) Chemical synthesis of restriction enzyme recognition sites useful for cloning. Science, 196 (4286). pp. 177-180. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20150630-151634552

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Abstract

By a triester chemical synthesis method, three decameric DNA's have been made; these act as substrates for several restriction endonucleases, including Eco RI, Bam I, and Hind III. These homogenous decamers form duplexes that can be efficiently blunt-end ligated to themselves or to other DNA molecules by the action of T4 DNA ligase and thus are useful tools for molecular cloning experiments.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.847463DOIArticle
http://www.sciencemag.org/content/196/4286/177PublisherArticle
http://www.jstor.org/stable/1743682JSTORArticle
Additional Information:© 1977 American Association for the Advancement of Science. Received 4 February 1977. We thank J. Rosenberg and W. Gilbert for their stimulating contributions to the development of the linker concept. We also thank A. S. Lee, H. Heyneker, and J. Shine for help in demonstrating some of the enzyme reactions discussed in this report; and L. Shively for technical help. Supported by NIH grants GM-12121 and HD-04420, NSF grants PCM75-05886 and GB-26517, and an NIH predoctoral traineeship to R.H.S. This is contribution No. 5457 from the Norman W. Church Laboratory of Chemical Biology, California Institute of Technology.
Funders:
Funding AgencyGrant Number
NIHGM-12121
NIHHD-04420
NSFPCM75-05886
NSFGB-26517
NIHUNSPECIFIED
Other Numbering System:
Other Numbering System NameOther Numbering System ID
Caltech Norman W. Church Laboratory of Chemical Biology5457
Record Number:CaltechAUTHORS:20150630-151634552
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150630-151634552
Official Citation:Scheller, R., Dickerson, R., Boyer, H., Riggs, A., & Itakura, K. (1977). Chemical synthesis of restriction enzyme recognition sites useful for cloning. Science, 196(4286), 177-180. doi: 10.1126/science.847463
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:58713
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:01 Jul 2015 15:10
Last Modified:13 Feb 2019 18:06

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