Chemical synthesis of restriction enzyme recognition sites useful for cloning

Scheller, Richard H. and Dickerson, Richard E. and Boyer, Herbert W. and Riggs, Arthur D. and Itakura, Keiichi (1977) Chemical synthesis of restriction enzyme recognition sites useful for cloning. Science, 196 (4286). pp. 177-180. ISSN 0036-8075. doi:10.1126/science.847463. https://resolver.caltech.edu/CaltechAUTHORS:20150630-151634552

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Abstract

By a triester chemical synthesis method, three decameric DNA's have been made; these act as substrates for several restriction endonucleases, including Eco RI, Bam I, and Hind III. These homogenous decamers form duplexes that can be efficiently blunt-end ligated to themselves or to other DNA molecules by the action of T4 DNA ligase and thus are useful tools for molecular cloning experiments.

Item Type:

Article

Related URLs:

URL	URL Type	Description
http://dx.doi.org/10.1126/science.847463	DOI	Article
http://www.sciencemag.org/content/196/4286/177	Publisher	Article
http://www.jstor.org/stable/1743682	JSTOR	Article

Additional Information:

© 1977 American Association for the Advancement of Science. Received 4 February 1977. We thank J. Rosenberg and W. Gilbert for their stimulating contributions to the development of the linker concept. We also thank A. S. Lee, H. Heyneker, and J. Shine for help in demonstrating some of the enzyme reactions discussed in this report; and L. Shively for technical help. Supported by NIH grants GM-12121 and HD-04420, NSF grants PCM75-05886 and GB-26517, and an NIH predoctoral traineeship to R.H.S. This is contribution No. 5457 from the Norman W. Church Laboratory of Chemical Biology, California Institute of Technology.

Funders:

Funding Agency	Grant Number
NIH	GM-12121
NIH	HD-04420
NSF	PCM75-05886
NSF	GB-26517
NIH	UNSPECIFIED

Other Numbering System:

Other Numbering System Name	Other Numbering System ID
Caltech Norman W. Church Laboratory of Chemical Biology	5457

Issue or Number:

4286

DOI:

10.1126/science.847463

Record Number:

CaltechAUTHORS:20150630-151634552

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20150630-151634552

Official Citation:

Scheller, R., Dickerson, R., Boyer, H., Riggs, A., & Itakura, K. (1977). Chemical synthesis of restriction enzyme recognition sites useful for cloning. Science, 196(4286), 177-180. doi: 10.1126/science.847463

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58713

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CaltechAUTHORS

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Deposited On:

01 Jul 2015 15:10

Last Modified:

10 Nov 2021 22:09

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