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Amino-terminal sequence analysis of the structural proteins of Sindbis virus

Bell, John R. and Hunkapiller, Michael W. and Hood, Leroy E. and Strauss, James H. (1978) Amino-terminal sequence analysis of the structural proteins of Sindbis virus. Proceedings of the National Academy of Sciences of the United States of America, 75 (6). pp. 2722-2726. ISSN 0027-8424. PMCID PMC392635. https://resolver.caltech.edu/CaltechAUTHORS:20150707-153924494

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Abstract

The structural proteins of Sindbis virus, an enveloped virus which belongs to the Togavirus family, have been subjected to automated Edman degradation using improved techniques. Extensive NHrterminal sequences of about 50 residues were determined for each of the two membrane glycoproteins. In both cases the NH_2 terminus of the molecule was found to be similar in composition to typical water-soluble proteins. The viral capsid protein was found to have a blocked a-amino group. This is consistent with other observations that viral proteins derived from the NH_2 terminus of precursor molecules are often blocked.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.jstor.org/stable/68314 JSTORArticle
http://dx.doi.org/10.1073/pnas.75.6.2722 DOIArticle
http://www.pnas.org/content/75/6/2722PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC392635/PubMed CentralArticle
ORCID:
AuthorORCID
Hood, Leroy E.0000-0001-7158-3678
Additional Information:© 1978 National Academy of Sciences. Communicated by N. H. Horowitz, April 7, 1978. The preliminary sequence data obtained by using the solid-phase instrument were obtained by Dr. W. J. Dreyer and his colleagues and we express our gratitude to him for this work. Edith M. Lenches and Mary S. Martin provided expert technical assistance in the growth of the virus and its purification. This work was supported by Grants GM 06965 and AI110793 from the National Institutes of Health and by Grant PCM 77-26728 from the National Science Foundation.
Funders:
Funding AgencyGrant Number
NIHGM 06965
NIHAI 110793
NSFPCM 77-26728
Subject Keywords:membrane glycoproteins; togaviruses; microsequencing
Issue or Number:6
PubMed Central ID:PMC392635
Record Number:CaltechAUTHORS:20150707-153924494
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150707-153924494
Official Citation:Bell, J. R., Hunkapiller, M. W., Hood, L. E., & Strauss, J. H. (1978). Amino-Terminal Sequence Analysis of the Structural Proteins of Sindbis Virus. Proceedings of the National Academy of Sciences of the United States of America, 75(6), 2722-2726. doi: 10.2307/68314
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:58799
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:08 Jul 2015 14:32
Last Modified:03 Oct 2019 08:40

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