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Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA

Campbell, Judith L. and Richardson, Charles C. and Studier, F. William (1978) Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA. Proceedings of the National Academy of Sciences of the United States of America, 75 (5). pp. 2276-2280. ISSN 0027-8424. PMCID PMC392535. https://resolver.caltech.edu/CaltechAUTHORS:20150708-140421957

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Abstract

Fragments of phage T7 DNA have been cloned in Escherichia coli by using the plasmid pMB9. Such cloned fragments are able to recombine with infecting phages, thus providing a means to integrate the physical and genetic maps of T7 DNA. Approximately 65% of the T7 DNA molecule has been found in clones so far, and analysis of these clones has mapped genes 12-17 with an accuracy of about 1% the total length of T7 DNA. At least some cloned segments can supply T7 functions to infecting phages.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.75.5.2276DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC392535/PubMed CentralArticle
Additional Information:© 1978 National Academy of Sciences. Communicated by Eugene P. Kennedy, March 7, 1978. We thank O. Ritter for able technical assistance. The portion of this work carried out at Harvard Medical School was supported by Grant NP-1G from the American Cancer Society. The portion of this work carried out at Brookhaven National Laboratory was under the auspices of the Department of Energy and was supported by National Institutes of Health Research Grant GM-21872.
Funders:
Funding AgencyGrant Number
American Cancer SocietyNP-1G
NIHGM-21872
Subject Keywords:physical mapping; Escherichia coli; pMB9 plasmid
Issue or Number:5
PubMed Central ID:PMC392535
Record Number:CaltechAUTHORS:20150708-140421957
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150708-140421957
Official Citation:Campbell, J. L., Richardson, C. C., & Studier, F. W. (1978). Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA. Proceedings of the National Academy of Sciences, 75(5), 2276-2280.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:58813
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:09 Jul 2015 22:13
Last Modified:03 Oct 2019 08:40

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