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A Genetic Locus Regulates the Expression of Tissue-Specific mRNAs from Multiple Transcription Units

Levy, David E. and Lerner, Richard A. and Wilson, Michael C. (1982) A Genetic Locus Regulates the Expression of Tissue-Specific mRNAs from Multiple Transcription Units. Proceedings of the National Academy of Sciences of the United States of America, 79 (19). pp. 5823-5827. ISSN 0027-8424.

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129 GIX- mice, unlike animals of the congeneic partner strain GIX+, do not express significant amounts of the retroviral antigens gp70 and p30. Evidence is presented indicating that the GIX phenotype is specified by a distinct regulatory gene acting on multiple transcription units to control the levels of accumulation of specific mRNA species. The steady-state levels of retroviral-homologous mRNA from the tissues of GIX+ and GIX- mice were examined by blot hybridization using as probes DNA fragments from cloned murine leukemia viruses. RNA potentially encoding viral antigens was reduced or absent in GIX- mice, even though no differences in integrated viral genomes were detected between these congeneic strains by DNA blotting. Tissue-specific patterns of accumulation of these RNA species were detected in brain, epididymis, liver, spleen, and thymus, and several distinct RNA species were found to be coordinately regulated with the GIX phenotype. Measurements of RNA synthesis suggest a major role for transcriptional control in the regulation of some retroviral messages.

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Additional Information:Copyright © 1982 by the National Academy of Sciences Communicated by Frank J. Dixon, June 23, 1982 We thank I. Verma, D. Lowy, and M. Harpold for providing recombinant DNA plasmids; M. Harpold and R. Ogata for critically reading the manuscript; R. Evans for communicating results prior to publication and for suggesting the RNA isolation procedure; P. Branks for expert technical assistance; and S. Baldwin for preparing the manuscript. Support was provided by the Department of Immunopathology, Research Institute of Scripps Clinic, and by U.S. Public Health Service Grant CA 27489 and Predoctoral Training Grant GM-07616. This is paper no. 2760 of the Research Institute of Scripps Clinic. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:endogenous retroviral gene; GIX phenotype; gp70; RNA blot hybridization; transcriptional control
Issue or Number:19
Record Number:CaltechAUTHORS:LEVpnas82b
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5924
Deposited By: Archive Administrator
Deposited On:08 Nov 2006
Last Modified:02 Oct 2019 23:27

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