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Vital labeling of embryonic cells using fluorescent dyes and proteins

Bhattacharyya, Sujata and Kulesa, Paul M. and Fraser, Scott E. (2008) Vital labeling of embryonic cells using fluorescent dyes and proteins. In: Avian Embryology, 2nd Edition. Methods in Cell Biology. No.87. Elsevier , New York, NY, pp. 187-210. ISBN 978-0-12-564174-6.

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This chapter presents techniques used for labeling single cells or small groups of cells with fluorescent dyes and proteins. Fluorescent dextran and photo-activatable fluorescent proteins (PAFP) labeling of cells offer a direct means to label single cells or small groups of cells, making it appropriate for either fate mapping or cell lineage studies. Lipid-soluble carbocyanine dyes offer a simpler means to label groups of cells for fate mapping studies. The experimental requirements for cell lineage and fate map studies are very similar. Both require a means of labeling a cell in a defined region of the embryo, of identifying the progeny of the labeled cell(s) over time, and of scoring the final phenotypes and positions of the progeny. There are a number of methods available to label single cells as well as small populations of cells in the chick embryo. While intracellular injection of rhodamine dextran is a very reliable technique for labeling individual cells, it is also a highly invasive technique. Consequently, cell death occurs in more than half the injected cells. 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine (DiI) on the other hand is less invasive but not noninvasive. It is best used for labeling more than one cell.

Item Type:Book Section
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Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2008 Elsevier.
Series Name:Methods in Cell Biology
Issue or Number:87
Record Number:CaltechAUTHORS:20150813-133334431
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Official Citation:Sujata Bhattacharyya, Paul M. Kulesa, Scott E. Fraser, Vital Labeling of Embryonic Cells Using Fluorescent Dyes and Proteins, Methods in Cell Biology, Academic Press, 2008, Volume 87, Pages 187-210, ISSN 0091-679X, ISBN 9780125641746, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:59500
Deposited By: George Porter
Deposited On:13 Aug 2015 22:10
Last Modified:03 Oct 2019 08:47

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