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Segregation of lens and olfactory precursors from a common territory: cell sorting and reciprocity of Dlx5 and Pax6 expression

Bhattacharyya, Sujata and Bailey, Andrew P. and Bronner-Fraser, Marianne and Streit, Andrea (2004) Segregation of lens and olfactory precursors from a common territory: cell sorting and reciprocity of Dlx5 and Pax6 expression. Developmental Biology, 271 (2). pp. 403-14. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20150813-133336793

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Abstract

Cranial placodes are focal regions of columnar epithelium next to the neural tube that contribute to sensory ganglia and organs in the vertebrate head, including the olfactory epithelium and the crystalline lens of the eye. Using focal dye labelling within the presumptive placode domain, we show that lens and nasal precursors arise from a common territory surrounding the anterior neural plate. They then segregate over time and converge to their final positions in discrete placodes by apparently directed movements. Since these events closely parallel the separation of eye and antennal primordia (containing olfactory sensory cells) from a common imaginal disc in Drosophila, we investigated whether the vertebrate homologues of Distalless (Dll) and Eyeless (Ey), which determine antennal and eye identity in the fly, play a role in segregation of lens and nasal precursors in the chick. Dlx5 and Pax6 are initially co-expressed by future lens and olfactory cells. As soon as presumptive lens cells acquire columnar morphology all Dlx family members are down-regulated in the placode, while Pax6 is lost in the olfactory region. Lens precursor cells that express ectopic Dlx5 never acquire lens-specific gene expression and are excluded from the lens placode to cluster in the head ectoderm. These results suggest that the loss of Dlx5 is required for cells to adopt a lens fate and that the balance of Pax6 and Dlx expression regulates cell sorting into appropriate placodal domains.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2004.04.010DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160604002799PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Streit, Andrea0000-0001-7664-7917
Additional Information:© 2004 Elsevier. Received for publication 21 April 2004, accepted 23 April 2004, Available online 25 May 2004. We thank Drs. A. Bang and D. Kosher for cDNA clones, Jhumku Kohtz and Joram Piatigorski for antibodies, and Heide Olsen for excellent technical assistance. We also thank Dan Darcy, Carole Lu, Ying Gong and Seth Ruffins for invaluable advice on confocal time-lapse movies and their analysis. We are grateful to Dr. C.D. Stern for critical reading of the manuscript. This work was supported by BBSRC 29/G13701 and a Royal Society grant to AS and by NS41070 to MBF. S.B. is supported by a Howard Hughes Predoctoral Fellowship and A.B. is supported by a Fight for Sight Studentship.
Funders:
Funding AgencyGrant Number
Biotechnology and Biological Sciences Research Council (BBSRC)29/G13701
Royal SocietyUNSPECIFIED
NIHNS41070
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Fight for Sight StudentshipUNSPECIFIED
Subject Keywords:Chick; Distal-less; Dlx; Eye; Eyeless; Fate map; Nasal epithelium; Pax6; Placode; Sensory organ
Issue or Number:2
Record Number:CaltechAUTHORS:20150813-133336793
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150813-133336793
Official Citation:Sujata Bhattacharyya, Andrew P. Bailey, Marianne Bronner-Fraser, Andrea Streit, Segregation of lens and olfactory precursors from a common territory: cell sorting and reciprocity of Dlx5 and Pax6 expression, Developmental Biology, Volume 271, Issue 2, 15 July 2004, Pages 403-414, ISSN 0012-1606, http://dx.doi.org/10.1016/j.ydbio.2004.04.010. (http://www.sciencedirect.com/science/article/pii/S0012160604002799)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:59503
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Aug 2015 21:35
Last Modified:09 Mar 2020 13:19

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