CaltechAUTHORS
  A Caltech Library Service

Primary steps of the photoactive yellow protein: Isolated chromophore dynamics and protein directed function

Lee, I-Ren and Lee, Wonchul and Zewail, Ahmed H. (2006) Primary steps of the photoactive yellow protein: Isolated chromophore dynamics and protein directed function. Proceedings of the National Academy of Sciences of the United States of America, 103 (2). pp. 258-262. ISSN 0027-8424. PMCID PMC1326191. https://resolver.caltech.edu/CaltechAUTHORS:LEEpnas06

[img]
Preview
PDF - Published Version
See Usage Policy.

472Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:LEEpnas06

Abstract

The cycle of the photoactive yellow protein (PYP) has been extensively studied, but the dynamics of the isolated chromophore responsible for transduction is unknown. Here, we present real-time observation of the dynamics of the negatively charged chromophore and detection of intermediates along the path of trans-to-cis isomerization using femtosecond mass selection/electron detachment techniques. The results show that the role of the protein environment is not in the first step of double-bond twisting (barrier crossing) but in directing efficient conversion to the cis-structure and in impeding radical formation within the protein.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.0510015103DOIArticle
http://www.pnas.org/content/103/2/258.longPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1326191/PubMed CentralArticle
http://dx.doi.org/OtherUNSPECIFIED
http://dx.doi.org/OtherUNSPECIFIED
Additional Information:© 2006 by The National Academy of Sciences of the USA Contributed by Ahmed H. Zewail, November 17, 2005. Published online before print January 3, 2006, 10.1073/pnas.0510015103 We thank Prof. Monique M. Martin and Drs. Agathe Espagne and D. Hern Paik for stimulating discussion; the collaborative work on solution-phase studies will be published elsewhere. This work was supported by the National Science Foundation. Author contributions: A.H.Z. designed research; I-R.L. and W.L. performed research; I-R.L. and W.L. analyzed data; and I-R.L., W.L., and A.H.Z. wrote the paper. Conflict of interest statement: No conflicts declared.
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
Subject Keywords:femtobiology; transduction; molecular dynamics; photoelectron spectroscopy
Issue or Number:2
PubMed Central ID:PMC1326191
Record Number:CaltechAUTHORS:LEEpnas06
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:LEEpnas06
Official Citation:I-Ren Lee, Wonchul Lee, and Ahmed H. Zewail Primary steps of the photoactive yellow protein: Isolated chromophore dynamics and protein directed function PNAS 2006 103 (2) 258-262; published ahead of print January 3, 2006, doi:10.1073/pnas.0510015103
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5968
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:09 Nov 2006
Last Modified:02 Oct 2019 23:28

Repository Staff Only: item control page