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New tools for visualization and analysis of morphogenesis in spherical embryos

Tyszka, J. Michael and Ewald, Andrew J. and Wallingford, John B. and Fraser, Scott E. (2005) New tools for visualization and analysis of morphogenesis in spherical embryos. Developmental Dynamics, 234 (4). pp. 974-983. ISSN 1058-8388. doi:10.1002/dvdy.20561. https://resolver.caltech.edu/CaltechAUTHORS:20150908-164249057

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Abstract

Many classical models of development, including amphibians, fish, and echinoderms, have embryos that are approximately spherical and contain concentric cell layers during early development. Neighbor relationships in such curved cell layers are not conveniently assayed or measured by conventional physical or optical sectioning techniques. To answer these challenges, we have constructed computational methods that correct for spherical distortion in 2D images and that allow extraction of concentric cell layers from 3D digital images. These methods for quantitative analysis and visualization of early development in spherical embryos are introduced by using them for the quantitative analysis of 2D and 3D images of gastrula stage Xenopus laevis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/dvdy.20561DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/dvdy.20561/abstractPublisherArticle
http://onlinelibrary.wiley.com/doi/10.1002/dvdy.20561/suppinfoPublisherSupporting Information
ORCID:
AuthorORCID
Tyszka, J. Michael0000-0001-9342-9014
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2005 Wiley-Liss, Inc. Received 14 February 2005; Revised 8 July 2005; Accepted 22 July 2005. Published online 28 September 2005. We thank Resolution Sciences Corporation (now Microscience Group, Inc.) for donating instrument time to image the frog embryos for this project; R. Kerschmann, M. Reddington, L. Garrett, P. Guthrie, M. Bolles, M. Haugh, and B. Herrera for technical assistance with SIM. We thank Sara M. Peyrot for providing the embryos shown in Figure 2. A.J.E. thanks Zena Werb for space and support during the writing of this manuscript. This work was funded in part by the Human Brain Project (EB00232) with contributions from the National Institute of Biomedical Imaging and Bioengineering and the National Institute of Mental Health, the National Center for Research Resources (RR13625), the National Institute of Mental Health MH61223, and by a National Institute of Childhood Diseases grant to S.E.F. A.J.E. was supported by the Burroughs Wellcome Fund Interfaces Program via the Caltech Initiative in Computational Molecular Biology. J.B.W. is supported by a Burroughs Wellcome Fund Career Award in the Biomedical Sciences.
Funders:
Funding AgencyGrant Number
National Institute of Biomedical Imaging and BioengineeringEB00232
National Center for Research Resources (NCRR)RR13625
National Institute of Mental Health (NIMH)MH61223
National Institute of Childhood DiseasesUNSPECIFIED
Burroughs Wellcome FundUNSPECIFIED
Subject Keywords:Xenopus laevis; early development; gastrulation; spherical geometry; numerical methods
Issue or Number:4
DOI:10.1002/dvdy.20561
Record Number:CaltechAUTHORS:20150908-164249057
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150908-164249057
Official Citation:Tyszka, J. M., Ewald, A. J., Wallingford, J. B. and Fraser, S. E. (2005), New tools for visualization and analysis of morphogenesis in spherical embryos. Dev. Dyn., 234: 974–983. doi: 10.1002/dvdy.20561
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:60110
Collection:CaltechAUTHORS
Deposited By: Julian Tyszka
Deposited On:10 Sep 2015 23:45
Last Modified:10 Nov 2021 22:29

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