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Statistical diffusion tensor histology reveals regional dysmyelination effects in the shiverer mouse mutant

Tyszka, J. Michael and Readhead, Carol and Bearer, Elaine L. and Pautler, Robia G. and Jacobs, Russell E. (2006) Statistical diffusion tensor histology reveals regional dysmyelination effects in the shiverer mouse mutant. NeuroImage, 29 (4). pp. 1058-1065. ISSN 1053-8119. PMCID PMC3376084.

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Shiverer is an important model of central nervous system dysmyelination characterized by a deletion in the gene encoding myelin basic protein with relevance to human dysmyelinating and demyelinating diseases. Perfusion fixed brains from shiverer mutant (C3Fe.SWV Mbp^(shi)/Mbp^(shi)n = 6) and background control (C3HeB.FeJ, n = 6) mice were compared using contrast enhanced volumetric diffusion tensor magnetic resonance microscopy with a nominal isotropic spatial resolution of 80 mum. Images were accurately coregistered using non-linear warping allowing voxel-wise statistical parametric mapping of tensor invariant differences between control and shiverer groups. Highly significant differences in the tensor trace and both the axial and radial diffusivity were observed within the major white matter tracts and in the thalamus, midbrain, brainstem and cerebellar white matter, consistent with a high density of myelinated axons within these regions. The fractional anisotropy was found to be much less sensitive than the trace and eigenvalues to dysmyelination and associated microanatomic changes.

Item Type:Article
Related URLs:
URLURL TypeDescription DOIArticle CentralArticle
Tyszka, J. Michael0000-0001-9342-9014
Jacobs, Russell E.0000-0002-1382-8486
Additional Information:© 2005 Elsevier Inc. Received 12 April 2005; revised 5 August 2005; accepted 23 August 2005; Available online 5 October 2005. The authors wish to thank Tim Hiltner for mouse perfusions and brain preparation, Natasha Kovacevic, Josette Chen and Mark Henkelmann for invaluable discussions regarding image coregistration and tissue fixation. This work was funded in part by the Human Brain Project (EB00232) with contributions from the National Institute of Biomedical Imaging and Bioengineering and the National Institute of Mental Health, the NCRR (RR13625) and NIHM (MH61223).
Funding AgencyGrant Number
Human Brain ProjectEB00232
National Institute of Biomedical Imaging and BioengineeringUNSPECIFIED
National Institute of Mental Health (NIMH)UNSPECIFIED
National Center for Research Resources (NCRR)RR13625
National Institute of Mental Health (NIMH)MH61223
Subject Keywords:Dysmyelination; Shiverer mutant; Magnetic resonance microscopy; Diffusion tensor imaging
Issue or Number:4
PubMed Central ID:PMC3376084
Record Number:CaltechAUTHORS:20150908-164452714
Persistent URL:
Official Citation:J. Michael Tyszka, Carol Readhead, Elaine L. Bearer, Robia G. Pautler, Russell E. Jacobs, Statistical diffusion tensor histology reveals regional dysmyelination effects in the shiverer mouse mutant, NeuroImage, Volume 29, Issue 4, 15 February 2006, Pages 1058-1065, ISSN 1053-8119, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:60112
Deposited By: Julian Tyszka
Deposited On:11 Sep 2015 04:47
Last Modified:03 Oct 2019 08:53

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