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A transgenic quail model that enables dynamic imaging of amniote embryogenesis

Huss, David and Benazeraf, Bertrand and Wallingford, Allison and Filla, Michael and Yang, Jennifer and Fraser, Scott E. and Lansford, Rusty (2015) A transgenic quail model that enables dynamic imaging of amniote embryogenesis. Development, 142 (16). pp. 2850-2859. ISSN 0950-1991. PMCID PMC4550965. https://resolver.caltech.edu/CaltechAUTHORS:20150911-095030467

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Abstract

Embryogenesis is the coordinated assembly of tissues during morphogenesis through changes in individual cell behaviors and collective cell movements. Dynamic imaging, combined with quantitative analysis, is ideal for investigating fundamental questions in developmental biology involving cellular differentiation, growth control and morphogenesis. However, a reliable amniote model system that is amenable to the rigors of extended, high-resolution imaging and cell tracking has been lacking. To address this shortcoming, we produced a novel transgenic quail that ubiquitously expresses nuclear localized monomer cherry fluorescent protein (chFP). We characterize the expression pattern of chFP and provide concrete examples of how Tg(PGK1:H2B-chFP) quail can be used to dynamically image and analyze key morphogenetic events during embryonic stages X to 11.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1242/dev.121392DOIArticle
http://dev.biologists.org/content/142/16/2850PublisherArticle
http://dev.biologists.org/content/142/16/2850/suppl/DC1PublisherSupplementary Material
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Lansford, Rusty0000-0002-2159-3699
Alternate Title:Transgenic quail to dynamically image amniote embryogenesis
Additional Information:© 2015 Published by The Company of Biologists Ltd. Received 4 January 2015; Accepted 5 July 2015; Posted online before print July 24, 2015. We thank Charlie Little and members of the R.L. lab for critically reading the manuscript. Competing interests: The authors declare no competing or financial interests. Author contributions: Conceived and designed the experiments: D.H., B.B. and R.L. Performed the experiments: D.H., B.B., A.W., J.Y., M.F. and R.L. Analyzed the data: D.H., B.B., S.E.F. and R.L. Wrote the paper: D.H., B.B. and R.L. Funding: This work was supported by grants from the National Institutes of Health (NIH) National Institute of General Medical Sciences [R01GM102801-01A], National Center for Research Resources [R21HD047347-01], National Human Genome Research Institute Centers of Excellence in Genomic Science program [P50 HG004071] and National Institute of Dental and Craniofacial Research FaceBase Consortium [U01-DE020063)]. M.F. was supported by the G. Harold & Leila Y. Mathers Charitable Foundation in the laboratory of Dr C. Little, University of Kansas Medical Center. Deposited in PMC for release after 12 months.
Funders:
Funding AgencyGrant Number
NIHR01GM102801-01A
National Center for Research Resources (NCRR)R21HD047347-01
National Human Genome Research InstituteP50 HG004071
National Institute of Dental and Craniofacial Research (NIDCR)U01-DE020063
G. Harold and Leila Y. Mathers Charitable FoundationUNSPECIFIED
Subject Keywords:Confocal microscopy, Quail embryo, Live imaging
Issue or Number:16
PubMed Central ID:PMC4550965
Record Number:CaltechAUTHORS:20150911-095030467
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150911-095030467
Official Citation:David Huss, Bertrand Benazeraf, Allison Wallingford, Michael Filla, Jennifer Yang, Scott E. Fraser, and Rusty Lansford A transgenic quail model that enables dynamic imaging of amniote embryogenesis Development 2015 142:2850-2859; posted ahead of print July 24, 2015, doi:10.1242/dev.121392
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:60187
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Sep 2015 18:21
Last Modified:03 Oct 2019 08:54

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