De Bellard, Maria Elena and Rao, Yi and Bronner-Fraser, Marianne (2003) Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells. Journal of Cell Biology, 162 (2). pp. 269-279. ISSN 0021-9525. PMCID PMC2172792. doi:10.1083/jcb.200301041. https://resolver.caltech.edu/CaltechAUTHORS:BELjcb03
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Abstract
Neural crest precursors to the autonomic nervous system form different derivatives depending upon their axial level of origin; for example, vagal, but not trunk, neural crest cells form the enteric ganglia of the gut. Here, we show that Slit2 is expressed at the entrance of the gut, which is selectively invaded by vagal, but not trunk, neural crest. Accordingly, only trunk neural crest cells express Robo receptors. In vivo and in vitro experiments demonstrate that trunk, not vagal, crest cells avoid cells or cell membranes expressing Slit2, thereby contributing to the differential ability of neural crest populations to invade and innervate the gut. Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells. These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.
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| Additional Information: | ©2003 The Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non- exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unpor ted license as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Submitted: 13 January 2003; revised: 11 June 2003; accepted: 16 June 2003. We thank Scott Fraser for helpful comments on the manuscript, Martin Garcia-Castro and Sara Ahlgren for help in analyzing the data, and Gustavo Gomez, Vivian Lee, and Joanne Tan-Cabugao for technical support. This work was supported in part by a postdoctoral fellowship to M.E. De Bellard from the National Multiple Sclerosis Society (FA 1383-A-1) and by a United States Public Health Service grant (HD-15527) to M. Bronner-Fraser. The supplemental material (Fig. S1 and Videos 1 and 2) is available at http://www.jcb.org/cgi/content/full/jcb.200301041/DC1. The movies show neural crest cells moving from three different experiments combined together for control and Slit2-exposed neural crest cells. The lapsed time is ~2.5 h. Videos 1 (4.2 MB) and 2 (8.3 MB) -- Slit2 enhances neural crest cell migration. Trunk neural tubes were cultured overnight on fibronectin. Cells were vitally labeled with CalceinAM (Molecular Probes) for confocal visualization after media was changed to one conditioned by control (HEK293 cells) (Video 2) or Slit2-secreting cells (Video 1) 1 h before video microscopy in a 410 LSM confocal for 3 h. Movies show three different experiments combined together for control and Slit2-exposed neural crest cells. | |||||||||
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| Subject Keywords: | Slit2; neural crest; vagal; chemorepellent; gut | |||||||||
| Issue or Number: | 2 | |||||||||
| PubMed Central ID: | PMC2172792 | |||||||||
| DOI: | 10.1083/jcb.200301041 | |||||||||
| Record Number: | CaltechAUTHORS:BELjcb03 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:BELjcb03 | |||||||||
| Official Citation: | Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells Maria Elena De Bellard, Yi Rao, and Marianne Bronner-Fraser J Cell Biol 2003 162:269-279. Published July 21, 2003, doi:10.1083/jcb.200301041 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 6019 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Lindsay Cleary | |||||||||
| Deposited On: | 14 Nov 2006 | |||||||||
| Last Modified: | 08 Nov 2021 20:30 |
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