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When Ends Meet: Circular DNA Stretches Differently in Elongational Flows

Li, Yanfei and Hsiao, Kai-Wen and Brockman, Christopher A. and Yates, Daniel Y. and Robertson-Anderson, Rae M. and Kornfield, Julia A. and San Francisco, Michael J. and Schroeder, Charles M. and McKenna, Gregory B. (2015) When Ends Meet: Circular DNA Stretches Differently in Elongational Flows. Macromolecules, 48 (16). pp. 5997-6001. ISSN 0024-9297. https://resolver.caltech.edu/CaltechAUTHORS:20150918-143504780

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Abstract

Chain topology has a profound impact on the flow behavior of single macromolecules. For circular polymers, the absence of free ends results in a unique chain architecture compared to linear or branched chains, thereby generating distinct molecular dynamics. Here, we report the direct observation of circular DNA dynamics in transient and steady flows for molecular sizes spanning the range of 25.0–114.8 kilobase pairs (kbp). Our results show that the longest relaxation times of the rings follow a power-law scaling relation with molecular weight that differs from that of linear chains. Also, relative to their linear counterparts, circular DNA molecules show a shifted coil-to-stretch transition and less diverse “molecular individualism” behavior as evidenced by their conformational stretching pathways. These results show the impact of chain topology on dynamics and reveal commonalities in the steady state behavior of circular and linear DNA that extends beyond chain architecture.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/acs.macromol.5b01374DOIArticle
http://pubs.acs.org/doi/10.1021/acs.macromol.5b01374PublisherArticle
ORCID:
AuthorORCID
Kornfield, Julia A.0000-0001-6746-8634
McKenna, Gregory B.0000-0002-5676-9930
Additional Information:© 2015 American Chemical Society. Received: June 23, 2015; Revised: July 25, 2015; Published: August 7, 2015. The work at TTU Chemical Engineering was supported by the John R. Bradford Endowment and the Paul Whitfield Horn Professorship. The work at UIUC was funded by a Dow Graduate Fellowship for K.-W.H. and C.A.B., and the David and Lucile Packard Foundation, NSF CAREER Award CBET-1254340, and the Camille and Henry Dreyfus Foundation for C.M.S. R.M.R.-A. was funded by the AFOSR Young Investigator Program, Grant No. FA95550-12-1-0315. The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
John R. Bradford EndowmentUNSPECIFIED
Paul Whitfield Horn ProfessorshipUNSPECIFIED
Dow Graduate FellowshipUNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
NSFCBET-1254340
Camille and Henry Dreyfus FoundationUNSPECIFIED
Air Force Office of Scientific Research (AFOSR)FA95550-12-1-0315
Issue or Number:16
Record Number:CaltechAUTHORS:20150918-143504780
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150918-143504780
Official Citation:When Ends Meet: Circular DNA Stretches Differently in Elongational Flows Yanfei Li, Kai-Wen Hsiao, Christopher A. Brockman, Daniel Y. Yates, Rae M. Robertson-Anderson, Julia A. Kornfield, Michael J. San Francisco, Charles M. Schroeder, and Gregory B. McKenna Macromolecules 2015 48 (16), 5997-6001 DOI: 10.1021/acs.macromol.5b01374
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:60343
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Sep 2015 01:04
Last Modified:09 Mar 2020 13:19

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