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Zebrafish TRPA1 Channels Are Required for Chemosensation But Not for Thermosensation or Mechanosensory Hair Cell Function

Prober, David A. and Zimmerman, Steven and Myers, Benjamin R. and McDermott, Brian M., Jr. and Kim, Seok-Hyung and Caron, Sophie and Rihel, Jason and Solnica-Krezel, Lilianna and Julius, David and Hudspeth, A. J. and Schier, Alexander F. (2008) Zebrafish TRPA1 Channels Are Required for Chemosensation But Not for Thermosensation or Mechanosensory Hair Cell Function. Journal of Neuroscience, 28 (40). pp. 10102-10110. ISSN 0270-6474. http://resolver.caltech.edu/CaltechAUTHORS:20150918-155039693

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Abstract

Transient receptor potential (TRP) ion channels have been implicated in detecting chemical, thermal, and mechanical stimuli in organisms ranging from mammals to Caenorhabditis elegans. It is well established that TRPA1 detects and mediates behavioral responses to chemical irritants. However, the role of TRPA1 in detecting thermal and mechanical stimuli is controversial. To further clarify the functions of TRPA1 channels in vertebrates, we analyzed their roles in zebrafish. The two zebrafish TRPA1 paralogs are expressed in sensory neurons and are activated by several chemical irritants in vitro. High-throughput behavioral analyses of trpa1a and trpa1b mutant larvae indicate that TRPA1b is necessary for behavioral responses to these chemical irritants. However, TRPA1 paralogs are not required for behavioral responses to temperature changes or for mechanosensory hair cell function in the inner ear or lateral line. These results support a role for zebrafish TRPA1 in chemical but not thermal or mechanical sensing, and establish a high-throughput system to identify genes and small molecules that modulate chemosensation, thermosensation, and mechanosensation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1523/JNEUROSCI.2740-08.2008DOIArticle
http://www.jneurosci.org/content/28/40/10102.abstractPublisherArticle
http://www.jneurosci.org/content/28/40/10102/suppl/DC1PublisherSupplemental Data
ORCID:
AuthorORCID
Prober, David A.0000-0002-7371-4675
Additional Information:© 2008 Society for Neuroscience. For the first six months after publication SfN’s license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received June 16, 2008. Revision received August 22, 2008. Accepted August 24, 2008. This work was supported by National Institutes of Health (NIH) Grants DC00241 (A.J.H.), NS055799 (D.J.), and NS049319 (A.F.S.); the Zebrafish Vanderbilt Initiative Academic Venture Capital Fund (L.S.-K.); the University Hospitals Case Medical Center−Center for Clinical Research and Technology (B.M.M.); and the Case Research Institute Vision Fund (B.M.M.). D.A.P. was supported by a fellowship from the Helen Hay Whitney Foundation and an NIH Pathway to Independence award. B.R.M. is a Predoctoral Fellow of the American Heart Association. B.M.M. is supported by a Basil O'Connor Starter Scholar Research Award from the March of Dimes. J.R. is a Bristol-Myers Squibb Life Sciences Research Foundation Fellow. A.J.H. is an Investigator of the Howard Hughes Medical Institute. We thank Drs. Cuppen, de Bruin, and Plasterk (Hubrecht laboratory) and Drs. Kettleborough and Stemple (Welcome Trust Sanger Institute) for providing the zebrafish trpa1a knock-out mutant (ZF-MODELS Integrated Project; contract number LSHG-CT-2003-503496; funded by the European Commission). We thank B. Fabella for providing the software for recording microphonic potentials, M. Vologodskaia and C. Fernando for genotyping larvae used in microphonic potential recordings and FM1-43 experiments, M. West for performing FM1-43 injections, and David Schoppik and Ian Woods for comments on this manuscript.
Funders:
Funding AgencyGrant Number
NIHDC00241
NIHNS055799
NIHNS049319
Zebrafish Vanderbilt Initiative Academic Venture Capital FundUNSPECIFIED
University Hospitals Case Medical Center−Center for Clinical Research and TechnologyUNSPECIFIED
Case Research Institute Vision FundUNSPECIFIED
Helen Hay Whitney FoundationUNSPECIFIED
NIH Pathway to Independence AwardUNSPECIFIED
American Heart Association (AHA) Predoctoral FellowshipUNSPECIFIED
March of DimesUNSPECIFIED
Life Sciences Research FoundationUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:nociception; temperature; mechanosensory; chemosensory; behavior; hair cell
Record Number:CaltechAUTHORS:20150918-155039693
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150918-155039693
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:60356
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:18 Sep 2015 23:15
Last Modified:18 Sep 2015 23:15

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