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Electron Microscope Studies of Heteroduplex DNA from a Deletion Mutant of Bacteriophage phi X-174

Kim, Jung-Suh and Sharp, Phillip A. and Davidson, Norman (1972) Electron Microscope Studies of Heteroduplex DNA from a Deletion Mutant of Bacteriophage phi X-174. Proceedings of the National Academy of Sciences of the United States of America, 69 (7). pp. 1948-1952. ISSN 0027-8424.

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A population of double-stranded replicative form of DNA molecules from bacteriophage phi X-174 carrying a deletion of about 9% of the wild-type DNA has been discovered in a sample cultivated under conditions where the phage lysozyme gene is nonessential. The structures of deleted monomers, dimers, and trimers were studied by the electron microscope heteroduplex method. The dimers and trimers are head-to-tail repeats of the deleted monomers. Some interesting examples of the dynamical phenomenon of branch migration in vitro have been observed in heteroduplexes of deleted dimer and trimer strands with undeleted monomer viral strands from the wild-type phage.

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Additional Information:Copyright © 1972 by the National Academy of Sciences Contributed by Norman Davidson, December 30, 1971 This research was supported by NIH Grant GM 10991. P.A.S. was supported by a USPHS fellowship. Our indebtedness to John Sedat is evident from the text. We wish also to acknowledge cordial interaction with A. Zuccarelli, R.L. Benbow, and R.L. Sinsheimer.
Subject Keywords:E. coli; phage lysozyme; length measurements; single-strand loops; DNA oligomers
Issue or Number:7
Record Number:CaltechAUTHORS:KIMpnas72
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6052
Deposited By: Archive Administrator
Deposited On:15 Nov 2006
Last Modified:02 Oct 2019 23:29

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