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Vectored antibody gene delivery mediates long-term contraception

Li, Juan and Olvera, Alejandra I. and Akbari, Omar S. and Moradian, Annie and Sweredoski, Michael J. and Hess, Sonja and Hay, Bruce A. (2015) Vectored antibody gene delivery mediates long-term contraception. Current Biology, 25 (19). R820-R822. ISSN 0960-9822. doi:10.1016/j.cub.2015.08.002. https://resolver.caltech.edu/CaltechAUTHORS:20151007-130607394

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Abstract

Development of non-surgical methods of long-term or permanent contraception remains a challenge. Towards this objective, we show that intramuscular injection of a replication-incompetent, recombinant adeno-associated virus (rAAV) designed to express an antibody that binds gonadotropin-releasing hormone (GnRH), a master regulator of reproduction in vertebrates, results in long-term infertility in male and female mice. Female mice are also rendered infertile through rAAV-dependent expression of an antibody that binds to the zona pellucida (ZP), a glycoprotein matrix that surrounds the egg and functions as a sperm-binding site. Many proteins known or suspected to be important for reproduction can be targeted, potentially reversibly, using this approach, which we refer to as vectored contraception (VC).


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cub.2015.08.002DOIArticle
ORCID:
AuthorORCID
Akbari, Omar S.0000-0002-6853-9884
Moradian, Annie0000-0002-0407-2031
Sweredoski, Michael J.0000-0003-0878-3831
Hess, Sonja0000-0002-5904-9816
Additional Information:© 2015 Elsevier Ltd. We thank Janet Baer and Karen Lencioni for providing advice on a number of aspects of this work relating to mouse husbandry and reproduction. Shirley Pease provided help with mouse superovulation and Ken Chan with microscopy of eggs. Alejandro B. Balazs and Rachel Galimidi provided help with AAV. Jost Vielmetter and the Caltech Protein Expression Center provided assistance with protein production and SPR measurements. Cynthia Besch-Williford (IDEXX Bioresearch) provided analysis of mouse histology. This work did not receive specific funding. The Proteome Exploration Laboratory (A.M., M.J.S., S.H.) was supported by the Gordon and Betty Moore Foundation through Grant GBMF775, the Beckman Institute, and the NIH, through Grant 1S10RR029594-01A1. A. Olvera was supported by a Gates Millennium Scholars award.
Funders:
Funding AgencyGrant Number
Gordon and Betty Moore FoundationGBMF775
Caltech Beckman InstituteUNSPECIFIED
NIH1S10RR029594-01A1
Bill and Melinda Gates FoundationUNSPECIFIED
Issue or Number:19
DOI:10.1016/j.cub.2015.08.002
Record Number:CaltechAUTHORS:20151007-130607394
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151007-130607394
Official Citation:Juan Li, Alejandra I. Olvera, Omar S. Akbari, Annie Moradian, Michael J. Sweredoski, Sonja Hess, Bruce A. Hay, Vectored antibody gene delivery mediates long-term contraception, Current Biology, Volume 25, Issue 19, 5 October 2015, Pages R820-R822, ISSN 0960-9822, http://dx.doi.org/10.1016/j.cub.2015.08.002. (http://www.sciencedirect.com/science/article/pii/S0960982215009446)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:60876
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Oct 2015 22:49
Last Modified:10 Nov 2021 22:40

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