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Visualization of cellulose synthases in Arabidopsis secondary cell walls

Watanabe, Y. and Meents, M. J. and McDonnell, L. M. and Barkwill, S. and Sampathkumar, A. and Cartwright, H. N. and Demura, T. and Ehrhardt, D. W. and Samuels, A. L. and Mansfield, S. D. (2015) Visualization of cellulose synthases in Arabidopsis secondary cell walls. Science, 350 (6257). pp. 198-203. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20151019-134150332

[img] PDF (Materials and Methods; Figs. S1 to S5; Tables S1 and S2; Captions for movies S1 to S6) - Supplemental Material
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[img] Video (QuickTime) (Movie S1- Distribution and motility of YFP::CESA7 in the cell membrane of VND7::GR induced seedlings at mid-development of secondary cell wall formation) - Supplemental Material
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[img] Video (AVI) (Movie S2 - Golgi and SmaCCs containing YFP::CESA7 rapidly move between and pause at domains of secondary cell wall formation) - Supplemental Material
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[img] Video (QuickTime) (Movie S3 - Coordination between YFP::CESA7 containing Golgi and SmaCCs can be transient) - Supplemental Material
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[img] Video (QuickTime) (Movie S4 - Delivery of at least two YFP::CESA7 containing complexes by a SmaCC) - Supplemental Material
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[img] Video (QuickTime) (Movie S5 - Loss of secondary cell wall CSC organization at the plasma membrane due to near complete depolymerization of microtubules with oryzalin treatment) - Supplemental Material
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[img] Video (QuickTime) (Movie S6 - Insertion events of secondary cell wall CSC complexes to the plasma membrane are unaffected by depolymerization of microtubules s by oryzalin treatment) - Supplemental Material
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Abstract

Cellulose biosynthesis in plant secondary cell walls forms the basis of vascular development in land plants, with xylem tissues constituting the vast majority of terrestrial biomass. We used plant lines that contained an inducible master transcription factor controlling xylem cell fate to quantitatively image fluorescently tagged cellulose synthase enzymes during cellulose deposition in living protoxylem cells. The formation of secondary cell wall thickenings was associated with a redistribution and enrichment of CESA7-containing cellulose synthase complexes (CSCs) into narrow membrane domains. The velocities of secondary cell wall–specific CSCs were faster than those of primary cell wall CSCs during abundant cellulose production. Dynamic intracellular of endomembranes, in combination with increased velocity and high density of CSCs, enables cellulose to be synthesized rapidly in secondary cell walls.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.aac7446DOIArticle
http://www.sciencemag.org/content/350/6257/198PublisherArticle
http://www.sciencemag.org/content/350/6257/198/suppl/DC1PublisherSupplemental Materials
ORCID:
AuthorORCID
Sampathkumar, A.0000-0003-1703-0137
Additional Information:© 2015 American Association for the Advancement of Science. Received 5 June 2015; accepted 26 August 2015. Arabidopsis plants containing RFP::TUB6 were the kind gift of C. Ambrose and G. O. Wasteneys. Supported by CREATE and Discovery grants (L.S. and S.D.M.); Canadian Natural Sciences and Engineering Research Council doctoral postgraduate scholarships (Y.W., M.J.M., and L.M.M.); NSF grant MCB-1158372 (D.W.E. and H.N.C.); and Japan Society for the Promotion of Science KAKENHI grants 24114002 and 25291062 (T.D.). We thank the technical support staff of the UBC Bioimaging Facility and R. White for statistical advice. The pTA7001 plasmid (VP16-GR vector) is available from N.-H. Chua of The Rockefeller University under a material transfer agreement; the VND7-GR line is available fromT.D.; and the GFP::CESA3 and YFP::CESA7 VND7-GR lines are freely available from L.S. or S.D.M. for noncommercial purposes under a materials transfer agreement on the GVG system with N.-H. Chua. Further data are reported in the supplementary materials.
Funders:
Funding AgencyGrant Number
CREATEUNSPECIFIED
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
NSFMCB-1158372
Japan Society for the Promotion of Science (JSPS)24114002
Japan Society for the Promotion of Science (JSPS)25291062
Issue or Number:6257
Record Number:CaltechAUTHORS:20151019-134150332
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151019-134150332
Official Citation:Visualization of cellulose synthases in Arabidopsis secondary cell walls Y. Watanabe, M. J. Meents, L. M. McDonnell, S. Barkwill, A. Sampathkumar, H. N. Cartwright, T. Demura, D. W. Ehrhardt, A.L. Samuels, and S. D. Mansfield Science 9 October 2015: 350 (6257), 198-203. [DOI:10.1126/science.aac7446]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:61260
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Oct 2015 21:03
Last Modified:09 Mar 2020 13:19

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