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Identification of Genes Uniquely Expressed in the Germ Line Tissues of the Jewel Wasp Nasonia vitripennis

Ferree, Patrick M. and Fang, Christopher and Mastrodimos, Mariah and Hay, Bruce A. and Amrhein, Henry and Akbari, Omar S. (2015) Identification of Genes Uniquely Expressed in the Germ Line Tissues of the Jewel Wasp Nasonia vitripennis. G3, 5 (12). pp. 2647-2653. ISSN 2160-1836. PMCID PMC4683638. doi:10.1534/g3.115.021386. https://resolver.caltech.edu/CaltechAUTHORS:20151019-135715761

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[img] MS Excel (Table S1 - Mapping Statistics. Mapping statistics for all samples indicating the total reads which map to junctions, exons, to multiple locations, uniquely and total locations mapped) - Supplemental Material
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[img] MS Excel (Table S2 - Gene expression) - Supplemental Material
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[img] MS Excel (Table S3 - Transcript expression) - Supplemental Material
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[img] MS Excel (Table S4 - Gene Ontology and PFAM domain enrichment of all genes that are expressed > 10 FPKM in the testes with Q<0.05) - Supplemental Material
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[img] MS Excel (Table S5 - Gene Ontology and PFAM domain enrichment of all genes that are expressed > 10 FPKM in the ovary with Q<0.05) - Supplemental Material
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[img] MS Excel (Table S6 - Gene Ontology and PFAM domain enrichment of all genes that are expressed > 10 FPKM in the male carcass with Q<0.05) - Supplemental Material
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[img] MS Excel (Table S7 - Gene Ontology and PFAM domain enrichment of all genes that are expressed > 10 FPKM in the female carcass with Q<0.05) - Supplemental Material
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[img] MS Excel (Table S8 - Testes-exclusive transcripts. Requirements: Expression = 0 in all samples other than testes; RPKM ≥ 1 in testes. Total number of genes = 338 ) - Supplemental Material
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[img] MS Excel (Table S9 - Ovary-exclusive transcripts. Requirements: Expression = 0 in all samples other than ovary; RPKM ≥ 1 in ovary. Total number of genes = 22 ) - Supplemental Material
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[img] MS Excel (Table S10 - Germline-exclusive expression. Requirements: Expression = 0 expression in both the male/female somatic carcass samples; RPKM ≥ 1 in testes and ovaries. Total number of genes = 40) - Supplemental Material
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[img] MS Excel (Table S11 - Meiosis related gene expression) - Supplemental Material
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[img] MS Excel (Table S12 - Centrosomal related gene expression) - Supplemental Material
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Abstract

The jewel wasp Nasonia vitripennis is a rising model organism for the study of haplo-diploid reproduction characteristic of hymenopteran insects, which include all wasps, bees and ants. We performed transcriptional profiling of the ovary, the female soma, and the male soma of N. vitripennis in order to complement a previously existing transcriptome of the wasp testis. These data were deposited into an open access genome browser for visualization of transcripts relative to their gene models. We used these data to identify the assemblies of genes uniquely expressed in the germ line tissues. We found that one hundred and fifty six protein-coding genes are exclusively expressed in the wasp testis compared to only twenty-two in the ovary. Of the testis-specific genes, eight are candidates for male-specific DNA packaging proteins known as protamines. We found very similar expression patterns of centrosome associated genes in the testis and ovary, arguing that de novo centrosome formation, a key process for development of unfertilized eggs into males, likely does not rely on large scale transcriptional differences between these tissues. In contrast, a number of meiosis-related genes show a bias toward testis-specific expression, despite the lack of true meiosis in N. vitripennis males. These patterns may reflect an unexpected complexity of male gamete production in the haploid males of this organism. Broadly these data add to the growing number of genomic and genetic tools available in N. vitripennis for addressing important biological questions in this rising insect model organism.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1534/g3.115.021386DOIArticle
http://www.g3journal.org/content/5/12/2647/suppl/DC1PublisherSupporting Information
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4683638/PubMed CentralArticle
ORCID:
AuthorORCID
Ferree, Patrick M.0000-0002-4315-4363
Mastrodimos, Mariah0000-0002-2057-9190
Hay, Bruce A.0000-0002-5486-0482
Amrhein, Henry0000-0002-4264-140X
Akbari, Omar S.0000-0002-6853-9884
Alternate Title:Identification of Genes Uniquely Expressed in the Germ-Line Tissues of the Jewel Wasp Nasonia vitripennis
Additional Information:Copyright © 2015 Ferree et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Received August 17, 2015. Accepted September 29, 2015. Early Online October 13, 2015. We thank Igor Antoscheckin for input on this manuscript. We also thank Tim Megraw for advice on centrosome orthologs. This work was supported by an NSF CAREER award (NSF1451839) to Patrick M. Ferree and an NIH K22 grant (5K22AI113060-02) to Omar S. Akbari.
Funders:
Funding AgencyGrant Number
NSF1451839
NIH5K22AI113060-02
Issue or Number:12
PubMed Central ID:PMC4683638
DOI:10.1534/g3.115.021386
Record Number:CaltechAUTHORS:20151019-135715761
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151019-135715761
Official Citation:Identification of Genes Uniquely Expressed in the Germ-Line Tissues of the Jewel Wasp Nasonia vitripennis Patrick M. Ferree, Christopher Fang, Mariah Mastrodimos, Bruce A. Hay, Henry Amrhein, and Omar S. Akbari G3 December 2015 5:2647-2653; Early Online October 13, 2015, doi:10.1534/g3.115.021386
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:61261
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Oct 2015 21:29
Last Modified:19 May 2022 18:06

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