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Comparison of Archaeal and Bacterial Diversity in Methane Seep Carbonate Nodules and Host Sediments, Eel River Basin and Hydrate Ridge, USA

Mason, Olivia U. and Case, David H. and Naehr, Thomas H. and Lee, Raymond W. and Thomas, Randal B. and Bailey, Jake V. and Orphan, Victoria J. (2015) Comparison of Archaeal and Bacterial Diversity in Methane Seep Carbonate Nodules and Host Sediments, Eel River Basin and Hydrate Ridge, USA. Microbial Ecology, 70 (3). pp. 766-784. ISSN 0095-3628. https://resolver.caltech.edu/CaltechAUTHORS:20151023-073758726

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Abstract

Anaerobic oxidation of methane (AOM) impacts carbon cycling by acting as a methane sink and by sequestering inorganic carbon via AOM-induced carbonate precipitation. These precipitates commonly take the form of carbonate nodules that form within methane seep sediments. The timing and sequence of nodule formation within methane seep sediments are not well understood. Further, the microbial diversity associated with sediment-hosted nodules has not been well characterized and the degree to which nodules reflect the microbial assemblage in surrounding sediments is unknown. Here, we conducted a comparative study of microbial assemblages in methane-derived authigenic carbonate nodules and their host sediments using molecular, mineralogical, and geochemical methods. Analysis of 16S rRNA gene diversity from paired carbonate nodules and sediments revealed that both sample types contained methanotrophic archaea (ANME-1 and ANME-2) and syntrophic sulfate-reducing bacteria (Desulfobacteraceae and Desulfobulbaceae), as well as other microbial community members. The combination of geochemical and molecular data from Eel River Basin and Hydrate Ridge suggested that some nodules formed in situ and captured the local sediment-hosted microbial community, while other nodules may have been translocated or may represent a record of conditions prior to the contemporary environment. Taken together, this comparative analysis offers clues to the formation regimes and mechanisms of sediment-hosted carbonate nodules.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/s00248-015-0615-6DOIArticle
http://link.springer.com/article/10.1007%2Fs00248-015-0615-6PublisherArticle
http://rdcu.be/ttbWPublisherFree ReadCube access
ORCID:
AuthorORCID
Orphan, Victoria J.0000-0002-5374-6178
Additional Information:© 2015 Springer Science+Business Media New York. Received: 29 November 2014; Accepted: 10 April 2015; Published online: 7 May 2015. Olivia U. Mason and David H. Case share co-first authorship. VO conceived of the study and collected the samples at sea. OM processed the samples and optimized DNA extraction, as well as TRFLP and clone library analyses. DC performed iTAG processing and analyses, beta diversity analyses, and was the coordinating author of the manuscript. TN provided XRD data, RL performed the isotopic composition analyses, JB provided thin section images, and RT performed the pore water geochemical measurements. DC, OM, and VO principally contributed to writing the manuscript. Three anonymous reviews provided constructive suggestions to improve the manuscript. Elizabeth Trembath-Reichert, Stephanie Connon, and Jeff Marlow helped with customization of the Silva115_NR99 database. Alexis Pasulka provided helpful discussion regarding ordination and statistical probing of microbial communities. Josh Steele also provided discussion on ecological statistics and aided with bench-top lab work. Benjamin Harrison helped with TRFLP data interpretation. Jeff Marlow provided useful feedback on the manuscript. The crew of the R/V Atlantis cruise AT-15-11, as well as the pilots of DSV Alvin dives AD4249 and 4256, aided in sample recovery at sea. Funding for this work was provided by a National Science Foundation grant (BIO-OCE 0825791) to VO and an early career grant by the United States Department of Energy, Office of Biological and Environmental Research (DE-SC0003940) to VO. This research was also supported by a grant from the NASA Astrobiology Institute (Award #NNA13AA92A) to VO. This is NAI-Life Underground Publication 009. DC was funded by a National Science Foundation Graduate Research Fellowship.
Funders:
Funding AgencyGrant Number
NSFOCE-0825791
Department of Energy (DOE)DE-SC0003940
NASANNA13AA92A
NSF Graduate Research FellowshipUNSPECIFIED
Other Numbering System:
Other Numbering System NameOther Numbering System ID
NAI-Life Underground009
Issue or Number:3
Record Number:CaltechAUTHORS:20151023-073758726
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151023-073758726
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:61456
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:26 Oct 2015 20:12
Last Modified:03 Oct 2019 09:08

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