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The DNA Binding Properties of the Parsley bZIP Transcription Factor CPRF4a Are Regulated by Light

Wellmer, Frank and Schäfer, Eberhard and Harter, Klaus (2001) The DNA Binding Properties of the Parsley bZIP Transcription Factor CPRF4a Are Regulated by Light. Journal of Biological Chemistry, 276 (9). pp. 6274-6279. ISSN 0021-9258. doi:10.1074/jbc.M007971200.

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The common plant regulatory factors (CPRFs) from parsley are transcription factors with a basic leucine zipper motif that bind to cis-regulatory elements frequently found in promoters of light-regulated genes. Recent studies have revealed that certain CPRF proteins are regulated in response to light by changes in their expression level and in their intracellular localization. Here, we describe an additional mechanism contributing to the light-dependent regulation of CPRF proteins. We show that the DNA binding activity of the factor CPRF4a is modulated in a phosphorylation-dependent manner and that cytosolic components are involved in the regulation of this process. Moreover, we have identified a cytosolic kinase responsible for CPRF4a phosphorylation. Modification of recombinant CPRF4a by this kinase, however, is insufficient to cause a full activation of the factor, suggesting that additional modifications are required. Furthermore, we demonstrate that the DNA binding activity of the factor is modified upon light treatment. The results of additional irradiation experiments suggest that this photoresponse is controlled by different photoreceptor systems. We discuss the possible role of CPRF4a in light signal transduction as well as the emerging regulatory network controlling CPRF activities in parsley.

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Additional Information:Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc. Received for publication, August 31, 2000, and in revised form, December 4, 2000. Originally published In Press as doi:10.1074/jbc.M007971200 on December 5, 2000 This work was supported in part by Deutsche Forschungsgemeinschaft Grant SFB 388 (to E. S. and K. H.) and Human Frontier Science Program Grant RG-43/97 (to K. H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Issue or Number:9
Record Number:CaltechAUTHORS:WELjbc01
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6149
Deposited By: Archive Administrator
Deposited On:27 Nov 2006
Last Modified:08 Nov 2021 20:31

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