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^(13)C NMR Studies of Butyric Fermentation in Clostridium kluyveri

Smith, Gary M. and Kim, Byung W. and Franke, Antoine A. and Roberts, John D. (1985) ^(13)C NMR Studies of Butyric Fermentation in Clostridium kluyveri. Journal of Biological Chemistry, 260 (25). pp. 13509-13512. ISSN 0021-9258.

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The fermentation of ^(13)C-labeled ethanol and acetate into butyrate and caproate by Clostridium kluyveri has been studied by using ^(13)C NMR. The pathway involves the conversion of both ethanol and acetate into acetyl coenzymes A, two of which condense to form CoA-linked precursors of butyrate. If butyryl-CoA is involved in the condensation, caproate is the ultimate product. ATP is produced from acetyl-CoA via the reactions catalyzed by phosphotransacetylase and acetate kinase with acetate, a required carbon source, as a co-product. In spectra of whole cells incubated with the labeled carbon sources, label from ethanol appears rapidly in acetate, which then reaches a lower, steady-state concentration due to its re-entry into the pathway. The rapid initial production of acetate indicates equally rapid production of ATP. Label from acetate appears in ethanol only if ethanol is already present, indicating that this process is one of isotopic equilibration rather than net synthesis of ethanol from acetate. The ratio of butyrate to caproate produced depends strongly on the initial ratio of ethanol to acetate in the medium. The relative rates of utilization of ethanol and acetate vary as the fermentation proceeds. ^(13)C-^(13)C coupling in the butyrate and caproate produced from [1-^(13)C]ethanol and [2-^(13)C]acetate can be used to determine if the acetyl-CoA molecules arising from ethanol and acetate enter the same pool or if they remain separated. The data are consistent with random mixing of the acetyl-CoA produced from the two carbon sources.

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Additional Information:© 1985 American Society of Biological. Received for publication, November 8, 1984. This work was supported by a grant from the Dairy Council of California and the National Dairy Council (to G. M. S.) and by National Science Foundation Grant CHE79-04262 (to J. D. R.). This paper is Contribution 7281 from the Gates and Crellin Laboratories, California Institute of Technology. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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Dairy Council of CaliforniaUNSPECIFIED
National Dairy CouncilUNSPECIFIED
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Caltech Gates and Crellin Laboratories of Chemistry7281
Issue or Number:25
Record Number:CaltechAUTHORS:20151028-094222892
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Official Citation:G M Smith, B W Kim, A A Franke, and J D Roberts 13C NMR studies of butyric fermentation in Clostridium kluyveri. J. Biol. Chem. 1985 260: 13509-12.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:61625
Deposited By: Ruth Sustaita
Deposited On:28 Oct 2015 16:57
Last Modified:03 Oct 2019 09:09

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