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A Study by Nitrogen-15 Nuclear Magnetic Resonance Spectroscopy of the State of Histidine in the Catalytic Triad of α-Lytic Protease

Bachovchin, William W. and Roberts, John D. (1978) A Study by Nitrogen-15 Nuclear Magnetic Resonance Spectroscopy of the State of Histidine in the Catalytic Triad of α-Lytic Protease. In: International Symposium on Chemistry of Natural Products. Vol.4. International Union of Pure and Applied Chemistry , London, UK, pp. 379-389. https://resolver.caltech.edu/CaltechAUTHORS:20151029-115032732

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Abstract

Hydrolytic cleavage of peptide bonds is an energetically· favorable reaction, but one that normally takes place very slowly at room temperature, even in the presence of rather strong acids or bases. It can be strongly catalyzed by many proteases, and much effort has been expended to determine how these have the ability to increase the rate of hydrolysis by a million-fold or more in neutral solutions. One of the types of proteases, the serine-protease family, is characterized by the presence at the active site of a "catalytic triad" comprised of the side-chain residues of serine, histidine and aspartic acid.


Item Type:Book Section
Additional Information:© 1978 International Union of Pure and Applied Chemistry. Supported by the Public Health Service, Research Grant No. GM-11072 from the Division of General Medical Sciences, and by the National Science Foundation. We are greatly indebted to Professor John H. Richards and Dr. Michael W. Hunkapiller for their unfailing interest and assistance throughout the course of this research, but we should emphasize that they are in no way responsible for its deficiencies either in the experiments or our interpretation of the results.
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Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM-11072
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Record Number:CaltechAUTHORS:20151029-115032732
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151029-115032732
Official Citation:A Study by Nitrogen-15 Nuclear Magnetic Resonance Spectroscopy of the State of Histidine in the Catalytic Triad of International Union of Pure and Applied Chemistry-Lytic Protease, Symposium Papers of IUPAC 11th International Symposium on Chemistry of Natural Products, Golden Sands, Bulgaria, Vol. 4, pp. 379-389 (1978).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:61714
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:04 Nov 2015 16:24
Last Modified:03 Oct 2019 09:10

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