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TRP channel mediated neuronal activation and ablation in freely behaving zebrafish

Chen, Shijia and Chiu, Cindy N. and McArthur, Kimberly L. and Fetcho, Joseph R. and Prober, David A. (2016) TRP channel mediated neuronal activation and ablation in freely behaving zebrafish. Nature Methods, 13 (2). pp. 147-150. ISSN 1548-7091. PMCID PMC4851460. https://resolver.caltech.edu/CaltechAUTHORS:20151030-151749046

[img] PDF - Accepted Version
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[img] Image (JPEG) (Supplementary Figure 1: Csn induces locomotor activity in embryos expressing TRPV1 in sensory neurons) - Supplemental Material
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[img] Image (JPEG) (Supplementary Figure 2: Increased temperatures induce locomotor activity and neuronal activity in embryos expressing TRPA1 in sensory neurons) - Supplemental Material
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[img] Image (JPEG) (Supplementary Figure 3: Menthol induces locomotor activity and neuronal activity in embryos expressing TRPM8 in sensory neurons) - Supplemental Material
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[img] Image (JPEG) (Supplementary Figure 4: Csn induces neuronal activity in embryos expressing TRPV1 in sensory neurons) - Supplemental Material
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[img] Image (JPEG) (Supplementary Figure 5: Csn can reversibly and repeatedly stimulate TRPV1–expressing Rohon–Beard sensory neurons) - Supplemental Material
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[img] Image (JPEG) (Supplementary Figure 6: Csn exposure changes the excitability of TRPV1–expressing Rohon–Beard neurons in exposed spinal cord) - Supplemental Material
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[img] Image (JPEG) (Supplementary Figure 7: Perfusion of Csn during whole–cell recording from intact embryos is associated with modest Rohon–Beard cell body depolarization but significantly higher average firing rates compared to DMSO vehicle alone) - Supplemental Material
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[img] Image (JPEG) (Supplementary Figure 8: Csn induces apoptosis in TRPV1–expressing cells exposed to high Csn levels but does not ablate sensory neurons that do not express TRPV1) - Supplemental Material
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[img] Image (JPEG) (Supplementary Figure 9: TRPV1–mediated activation and ablation of Hcrt neurons affects sleep–wake behaviors) - Supplemental Material
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[img] Image (JPEG) (Supplementary Figure 10: Csn does not affect WT larval zebrafish behavior) - Supplemental Material
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[img] Video (MPEG) (Video 1: Behavioral response to Csn activation of TRPV1–expressing sensory neurons at 24 hpf) - Supplemental Material
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[img] Video (MPEG) (Video 2: Behavioral response to Csn activation of TRPV1–expressing sensory neurons at 48 hpf) - Supplemental Material
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[img] Video (MPEG) (Video 3: Behavioral response to thermal activation of TRPA1–expressing sensory neurons at 24 hpf) - Supplemental Material
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[img] Video (MPEG) (Video 4: Behavioral response to menthol activation of TRPM8–expressing sensory neurons at 24 hpf) - Supplemental Material
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[img] PDF (Supplementary Figures 1–10 and Supplementary Discussion) - Supplemental Material
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[img] Archive (ZIP) (Supplementary Data. Plasmid sequence files) - Supplemental Material
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Abstract

The zebrafish (Danio rerio) is a useful vertebrate model system in which to study neural circuits and behavior, but tools to modulate neurons in freely behaving animals are limited. As poikilotherms that live in water, zebrafish are amenable to thermal and pharmacological perturbations. We exploit these properties by using transient receptor potential (TRP) channels to activate or ablate specific neuronal populations using the chemical and thermal agonists of heterologously expressed TRPV1, TRPM8 and TRPA1.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/nmeth.3691DOIArticle
https://rdcu.be/gob5PublisherFree ReadCube access
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4851460PubMed CentralArticle
ORCID:
AuthorORCID
Prober, David A.0000-0002-7371-4675
Additional Information:© 2015 Nature Publishing Group. Received 03 September 2015; Accepted 09 November 2015; Published online 14 December 2015. We thank A. Chen, A. Cruz, A. Dominguez, J. Engle, S. Khan, K. Molinder, B. Niles, V. Sapin and L. Wang for technical assistance. This work was supported by grants from the US National Institutes of Health (F31-NS07842A to S.C.; F32-NS083099 to K.L.M.; R01-NS26539 and DP1-OD006411 to J.R.F.; R00-NS060996, R01-NS070911 and R01-DA031367 to D.A.P.) and the Mallinckrodt Foundation, the Rita Allen Foundation and the Brain and Behavior Research Foundation (D.A.P.). Author Contributions: D.A.P. conceived of and supervised the project. S.C. and C.N.C. designed, performed, and analyzed all experiments, except physiology experiments, which were designed, performed and analyzed by K.L.M. and J.R.F. All authors collaborated to write the manuscript. The authors declare no competing financial interests.
Funders:
Funding AgencyGrant Number
NIHF31-NS07842A
NIHF32-NS083099
NIHR01-NS26539
NIHDP1-OD006411
NIHR00-NS060996
NIHR01-NS070911
NIHR01-DA031367
Mallinckrodt FoundationUNSPECIFIED
Rita Allen FoundationUNSPECIFIED
Brain and Behavior Research FoundationUNSPECIFIED
Issue or Number:2
PubMed Central ID:PMC4851460
Record Number:CaltechAUTHORS:20151030-151749046
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151030-151749046
Official Citation:Chen, S., Chiu, C., McArthur, K. et al. TRP channel mediated neuronal activation and ablation in freely behaving zebrafish. Nat Methods 13, 147–150 (2016). https://doi.org/10.1038/nmeth.3691
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:61752
Collection:CaltechAUTHORS
Deposited By: Joy Painter
Deposited On:05 Jan 2016 22:30
Last Modified:14 Apr 2020 19:50

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