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Methane metabolism in the archaeal phylum Bathyarchaeota revealed by genome-centric metagenomics

Evans, Paul N. and Parks, Donovan H. and Chadwick, Grayson L. and Robbins, Steven J. and Orphan, Victoria J. and Golding, Suzanne D. and Tyson, Gene W. (2015) Methane metabolism in the archaeal phylum Bathyarchaeota revealed by genome-centric metagenomics. Science, 350 (6259). pp. 434-438. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20151103-081330300

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Abstract

Methanogenic and methanotrophic archaea play important roles in the global flux of methane. Culture-independent approaches are providing deeper insight into the diversity and evolution of methane-metabolizing microorganisms, but, until now, no compelling evidence has existed for methane metabolism in archaea outside the phylum Euryarchaeota. We performed metagenomic sequencing of a deep aquifer, recovering two near-complete genomes belonging to the archaeal phylum Bathyarchaeota (formerly known as the Miscellaneous Crenarchaeotal Group). These genomes contain divergent homologs of the genes necessary for methane metabolism, including those that encode the methyl–coenzyme M reductase (MCR) complex. Additional non-euryarchaeotal MCR-encoding genes identified in a range of environments suggest that unrecognized archaeal lineages may also contribute to global methane cycling. These findings indicate that methane metabolism arose before the last common ancestor of the Euryarchaeota and Bathyarchaeota.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.aac7745DOIArticle
http://www.sciencemag.org/content/350/6259/434PublisherArticle
http://www.sciencemag.org/content/350/6259/434/suppl/DC1PublisherSupplemental Materials
ORCID:
AuthorORCID
Chadwick, Grayson L.0000-0003-0700-9350
Orphan, Victoria J.0000-0002-5374-6178
Tyson, Gene W.0000-0001-8559-9427
Additional Information:© 2015 American Association for the Advancement of Science. Received 11 June 2015; accepted 14 September 2015. We thank E. Gagen and P. Hugenholtz for valuable comments and suggestions; K. Baublys, SGS-Leeder, and Australian Laboratory Services staff for sample collection; and M. Butler and S. Low for library preparation and sequencing. This study was supported by the Australian Research Council (ARC) Linkage Project (grant LP100200730) and the U.S. Department of Energy’s Office of Biological Environmental Research (award no. DE-SC0010574). D.H.P. is supported by the Natural Sciences and Engineering Research Council of Canada. S.J.R. is supported by an Australian Postgraduate Award Industry scholarship. G.W.T. is supported by an ARC Queen Elizabeth II Fellowship (grant DP1093175). The authors declare no conflicts of interest. Our Whole Genome Shotgun projects have been deposited in the DNA DataBank of Japan, the European Molecular Biology Laboratory repository, and NIH’s GenBank under the accession numbers LIHJ00000000 (BA1) and LIHK00000000 (BA2). The versions described in this paper are LIHJ01000000 (BA1) and LIHK01000000 (BA2). Non-euryarchaeotal Surat Basin mcrA sequences have been deposited under the accession numbers KT387805 to KT387832, and unprocessed reads have been deposited under the accession number SRX1122679.
Funders:
Funding AgencyGrant Number
Australian Research CouncilLP100200730
Department of Energy (DOE)DE-SC0010574
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
Australian Postgraduate Award Industry ScholarshipUNSPECIFIED
Australian Research CouncilDP1093175
Issue or Number:6259
Record Number:CaltechAUTHORS:20151103-081330300
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151103-081330300
Official Citation:Methane metabolism in the archaeal phylum Bathyarchaeota revealed by genome-centric metagenomics Paul N. Evans, Donovan H. Parks, Grayson L. Chadwick, Steven J. Robbins, Victoria J. Orphan, Suzanne D. Golding, and Gene W. Tyson Science 23 October 2015: 350 (6259), 434-438. [DOI:10.1126/science.aac7745]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:61783
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Nov 2015 16:27
Last Modified:03 Oct 2019 19:19

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