CaltechAUTHORS
  A Caltech Library Service

Chemomimetic Biocatalysis: Exploiting the Synthetic Potential of Cofactor-Dependent Enzymes To Create New Catalysts

Prier, Christopher K. and Arnold, Frances H. (2015) Chemomimetic Biocatalysis: Exploiting the Synthetic Potential of Cofactor-Dependent Enzymes To Create New Catalysts. Journal of the American Chemical Society, 137 (44). pp. 13992-14006. ISSN 0002-7863. http://resolver.caltech.edu/CaltechAUTHORS:20151105-083025660

[img] PDF (ACS AuthorChoice) - Published Version
See Usage Policy.

4Mb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20151105-083025660

Abstract

Despite the astonishing breadth of enzymes in nature, no enzymes are known for many of the valuable catalytic transformations discovered by chemists. Recent work in enzyme design and evolution, however, gives us good reason to think that this will change. We describe a chemomimetic biocatalysis approach that draws from small-molecule catalysis and synthetic chemistry, enzymology, and molecular evolution to discover or create enzymes with non-natural reactivities. We illustrate how cofactor-dependent enzymes can be exploited to promote reactions first established with related chemical catalysts. The cofactors can be biological, or they can be non-biological to further expand catalytic possibilities. The ability of enzymes to amplify and precisely control the reactivity of their cofactors together with the ability to optimize non-natural reactivity by directed evolution promises to yield exceptional catalysts for challenging transformations that have no biological counterparts.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://pubs.acs.org/doi/abs/10.1021/jacs.5b09348PublisherArticle
http://dx.doi.org/10.1021/jacs.5b09348DOIArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2015 American Chemical Society. ACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. Received: September 3, 2015; Published: October 26, 2015. Our research is supported by the National Science Foundation, Office of Chemical, Bioengineering, Environmental and Transport Systems SusChEM Initiative (grant CBET-1403077) and by the Division of Molecular and Cellular Biosciences (grant MCB-1513007). C.K.P. thanks the Resnick Sustainability Institute for a postdoctoral fellowship. We thank Drs. Donald Hilvert, Jared Lewis, Gerrit Poelarends, Andrew Buller, Sheel Dodani, Hans Renata, and David Romney for helpful suggestions.
Group:Resnick Sustainability Institute
Funders:
Funding AgencyGrant Number
NSFCBET-1403077
NSFMCB-1513007
Record Number:CaltechAUTHORS:20151105-083025660
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20151105-083025660
Official Citation:Chemomimetic Biocatalysis: Exploiting the Synthetic Potential of Cofactor-Dependent Enzymes To Create New Catalysts Christopher K. Prier and Frances H. Arnold Journal of the American Chemical Society 2015 137 (44), 13992-14006 DOI: 10.1021/jacs.5b09348
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:61871
Collection:CaltechAUTHORS
Deposited By: Heidi Rusina
Deposited On:05 Nov 2015 18:45
Last Modified:08 Sep 2017 22:51

Repository Staff Only: item control page