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A Mammalian Mediator Subunit that Shares Properties with Saccharomyces cerevisiae Mediator Subunit Cse2

Tomomori-Sato, Chieri and Sato, Shigeo and Parmely, Tari J. and Banks, Charles A. S. and Sorokina, Irina and Florens, Laurence and Zybailov, Boris and Washburn, Michael P. and Brower, Christopher S. and Conaway, Ronald C. and Conaway, Joan Weliky (2004) A Mammalian Mediator Subunit that Shares Properties with Saccharomyces cerevisiae Mediator Subunit Cse2. Journal of Biological Chemistry, 279 (7). pp. 5846-5851. ISSN 0021-9258. doi:10.1074/jbc.M312523200.

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The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. We previously identified and partially purified a mammalian Mediator complex from rat liver nuclei (Brower, C.S., Sato, S., Tomomori-Sato, C., Kamura, T., Pause, A., Stearman, R., Klausner, R.D., Malik, S., Lane, W.S., Sorokina, I., Roeder, R.G., Conaway, J.W., and Conaway, R.C. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 10353-10358). Analysis by tandem mass spectrometry of proteins present in the most highly purified rat Mediator fractions led to the identification of a collection of new mammalian Mediator subunits, as well as several potential Mediator subunits including a previously uncharacterized protein encoded by the FLJ10193open reading frame. In this study, we present direct biochemical evidence that the FLJ10193protein, which we designate Med25, is a bona fide subunit of the mammalian Mediator complex. In addition, we present evidence that Med25 shares structural and functional properties with Saccharomyces cerevisiae Mediator subunit Cse2 and may be a mammalian Cse2 ortholog. Taken together, our findings identify a novel mammalian Mediator subunit and shed new light on the architecture of the mammalian Mediator complex.

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Additional Information:© 2004 the American Society for Biochemistry and Molecular Biology. Received for publication, November 16, 2003 , and in revised form, November 24, 2003. Originally published In Press as doi:10.1074/jbc.M312523200 on November 24, 2003. We thank W. S. Lane for assistance with mass spectrometry. We also thank R. G. Roeder and S. Malik for the HeLa cell line stably expressing FLAG-Nut2. This work was supported by National Institutes of Health Grant R37 GM41628. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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NIHR37 GM41628
Issue or Number:7
Record Number:CaltechAUTHORS:TOMjbc04
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6207
Deposited By: Archive Administrator
Deposited On:28 Nov 2006
Last Modified:08 Nov 2021 20:32

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