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Structural Basis for a Broad But Selective Ligand Spectrum of a Mouse Olfactory Receptor: Mapping the Odorant-Binding Site

Katada, Sayako and Hirokawa, Takatsugu and Oka, Yuki and Suwa, Makiko and Touhara, Kazushige (2005) Structural Basis for a Broad But Selective Ligand Spectrum of a Mouse Olfactory Receptor: Mapping the Odorant-Binding Site. Journal of Neuroscience, 25 (7). pp. 1806-1815. ISSN 0270-6474. https://resolver.caltech.edu/CaltechAUTHORS:20151118-134439010

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Abstract

The olfactory receptor (OR) superfamily provides a basis for the remarkable ability to recognize and discriminate a large number of odorants. In mice, the superfamily includes ∼1000 members, and they recognize overlapping sets of odorants with distinct affinities and specificities. To address the molecular basis of odor discrimination by the mammalian OR superfamily, we performed functional analysis on a series of site-directed mutants and performed ligand docking simulation studies to define the odorant-binding site of a mouse OR. Our results indicate that several amino acids in the transmembrane domains formed a ligand-binding pocket. Although other G-protein-coupled receptors (GPCRs) recognize biogenic ligands mainly with ionic or hydrogen bonding interactions, ORs recognize odorants mostly via hydrophobic and van der Waals interactions. This accounts for the broad but selective binding by ORs as well as their relatively low ligand-binding affinities. Furthermore, we succeeded in rational receptor design, inserting point mutations in the odorant-binding site that resulted in predicted changes in ligand specificity and antagonist activity. This ability to rationally design the receptor validated the binding site structure that was deduced with our mutational and ligand docking studies. Such broad and specific sensitivity suggests an evolutionary process during which mutations in the active site led to an enormous number of ORs with a wide range of ligand specificity. The current study reveals the molecular environment of the odorant-binding site, and it further advances the understanding of GPCR pharmacology.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1523/JNEUROSCI.4723-04.2005DOIArticle
http://www.jneurosci.org/content/25/7/1806.longPublisherArticle
Additional Information:© 2005 Society for Neuroscience. For the first six months after publication SfN’s license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received Nov. 18, 2004; revised Dec. 18, 2004; accepted Dec. 21, 2004. This work was supported in part by the Program for Promotion of Basic Research Activities for Innovative Biosciences Japan (K.T.) and by the grant-in-aid for special projects in genome science (genome informatics) from the Ministry of Education, Sports, Science and Technology (T.H., M.S.). K.T. is a recipient of grants from the Kato Memorial Bioscience Foundation and the Uehara Memorial Foundation. We thank K. Kajiya for being involved in the initial stage of this project and members of the Touhara laboratory for helpful discussion.
Funders:
Funding AgencyGrant Number
PROBRAIN JapanUNSPECIFIED
Ministry of Education, Culture, Sports, Science and Technology (MEXT)UNSPECIFIED
Kato Memorial Bioscience FoundationUNSPECIFIED
Uehara Memorial FoundationUNSPECIFIED
Subject Keywords:computer modeling; G-protein-coupled receptor; ligand binding; mutation; odorant; olfactory receptor
Issue or Number:7
Record Number:CaltechAUTHORS:20151118-134439010
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151118-134439010
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:62209
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:18 Nov 2015 23:29
Last Modified:03 Oct 2019 09:16

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