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The Taste of Carbonation

Chandrashekar, Jayaram and Yarmolinsky, David and von Buchholtz, Lars and Oka, Yuki and Sly, William and Ryba, Nicholas J. P. and Zuker, Charles S. (2009) The Taste of Carbonation. Science, 326 (5951). pp. 443-445. ISSN 0036-8075. PMCID PMC3654389. http://resolver.caltech.edu/CaltechAUTHORS:20151118-134439277

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Abstract

Carbonated beverages are commonly available and immensely popular, but little is known about the cellular and molecular mechanisms underlying the perception of carbonation in the mouth. In mammals, carbonation elicits both somatosensory and chemosensory responses, including activation of taste neurons. We have identified the cellular and molecular substrates for the taste of carbonation. By targeted genetic ablation and the silencing of synapses in defined populations of taste receptor cells, we demonstrated that the sour-sensing cells act as the taste sensors for carbonation, and showed that carbonic anhydrase 4, a glycosylphosphatidylinositol-anchored enzyme, functions as the principal CO_2 taste sensor. Together, these studies reveal the basis of the taste of carbonation as well as the contribution of taste cells in the orosensory response to CO_2.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.1174601DOIArticle
http://www.sciencemag.org/content/326/5951/443PublisherArticle
http://www.sciencemag.org/content/326/5951/443/suppl/DC1PublisherSupporting Material
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654389/PubMed CentralArticle
Additional Information:© 2009 American Association for the Advancement of Science. Received for publication 6 April 2009. Accepted for publication 17 August 2009. We thank W. Guo and A. Becker for generation and maintenance of mouse lines, M. Hoon for help in the initial phase of this work, E. R. Swenson for a generous gift of benzolamide, M. Goulding for Rosa26-flox-STOP-TeNT mice, A. Waheed for Car4 antibodies, and members of the Zuker laboratory for valuable comments. Supported in part by the intramural research program of the NIH, NIDCR (N.J.P.R.). C.S.Z. is an investigator of the Howard Hughes Medical Institute.
Funders:
Funding AgencyGrant Number
National Institute of Dental and Craniofacial Research (NIDCR)UNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
PubMed Central ID:PMC3654389
Record Number:CaltechAUTHORS:20151118-134439277
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20151118-134439277
Official Citation:The Taste of Carbonation Jayaram Chandrashekar, David Yarmolinsky, Lars von Buchholtz, Yuki Oka, William Sly, Nicholas J. P. Ryba, and Charles S. Zuker Science 16 October 2009: 326 (5951), 443-445. [DOI:10.1126/science.1174601]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:62210
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:18 Nov 2015 23:32
Last Modified:19 Nov 2015 20:11

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