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Conformations of the Single-Stranded DNA of Bacteriophage M13

Forsheit, Arleen B. and Ray, Dan S. (1970) Conformations of the Single-Stranded DNA of Bacteriophage M13. Proceedings of the National Academy of Sciences of the United States of America, 67 (3). pp. 1534-1541. ISSN 0027-8424. PMCID PMC283387. https://resolver.caltech.edu/CaltechAUTHORS:20151120-084321090

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Abstract

At least two conformations of M13 single-stranded DNA have been demonstrated by measuring differences in sedimentation coefficient and by direct visualization in the electron microscope. Which form is obtained from infected cells and/or intact phage depends on the pH, ionic strength, and temperature. The slower-sedimenting form can be converted to the faster-sedimenting, single-stranded form by low ionic strength, alkali treatment, formamide, or formaldehyde, but not by exposure to 100°C in 1.0 M NaCl. The ability to assume either conformation appears to be a function of the nucleic acid alone. Whether or not these different conformations are of biological significance is still unknown.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.67.3.1534 DOIArticle
http://www.pnas.org/content/67/3/1534PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283387/PubMed CentralArticle
Additional Information:© 1970 National Academy of Sciences. Communicated by Norman Davidson, August 28, 1970. Supported by NSF grant GB 18074, University of California Cancer Research Funds, and an NSF predoctoral traineeship to A.B.F.
Funders:
Funding AgencyGrant Number
NSFGB 18074
University of California Cancer Research FundsUNSPECIFIED
NSF Predoctoral FellowshipUNSPECIFIED
Issue or Number:3
PubMed Central ID:PMC283387
Record Number:CaltechAUTHORS:20151120-084321090
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151120-084321090
Official Citation:Arleen B. Forsheit and Dan S. Ray Conformations of the Single-Stranded DNA of Bacteriophage M13 PNAS 1970 67 (3) 1534-1541
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:62271
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:20 Nov 2015 19:40
Last Modified:03 Oct 2019 09:17

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