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Assembly of Phage Lambda in Vitro

Weigle, J. (1966) Assembly of Phage Lambda in Vitro. Proceedings of the National Academy of Sciences of the United States of America, 55 (6). pp. 1462-1466. ISSN 0027-8424.

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Edgar and Wood [1] have shown recently that it is possible to assemble, in vitro, the parts of phage T4 which accumulate in mutant infected cells to form infectious, viable phage particles. This complementation in vitro can also be demonstrated for phage lambda, as is shown below. One would expect lambda to be less complex in structure than T4, since the chromosome of lambda has only one fourth of the DNA content of T4 and presumably contains fewer genes. Indeed, it is found that the physiological functions of the genes of lambda, as revealed by in vitro assemblage, divide into two groups only-head donors and tail donors, in the terminology of Edgar and Wood [1]. Evidence is presented which suggests that the heads and tails of lambda can unite to form viable phage in the absence of other factors.

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Additional Information:Copyright © 2005 by the National Academy of Sciences Communicated by Seymour Benzer, April 25, 1966 The author wishes to thank Dr. R. S. Edgar for his help in the redaction of the manuscript and for his daily critical and constructive advice, and Mr. R. J. Huskey who helped prepare the lysogenic bacteria. This research was supported by grants from the National Science Foundation (GB 3930) and from the U. S. Public Health Service (GM 06965).
Issue or Number:6
Record Number:CaltechAUTHORS:WEIpnas66
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6234
Deposited By: Archive Administrator
Deposited On:29 Nov 2006
Last Modified:02 Oct 2019 23:30

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