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Nucleotide Sequence of the 26S mRNA of Sindbis Virus and Deduced Sequence of the Encoded Virus Structural Proteins

Rice, Charles M. and Strauss, James H. (1981) Nucleotide Sequence of the 26S mRNA of Sindbis Virus and Deduced Sequence of the Encoded Virus Structural Proteins. Proceedings of the National Academy of Sciences of the United States of America, 78 (4). pp. 2062-2066. ISSN 0027-8424. PMCID PMC319283.

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The nucleotide sequence of intracellular 26S mRNA of Sindbis virus has been determined by direct sequence analysis of the cDNA made to this RNA with reverse transcriptase. From this, the amino acid sequences of the encoded virus structural proteins, which include a basic capsid protein and two integral membrane glycoproteins, have been deduced. The features of these proteins as related to their functions are discussed. We suggest that three proteases are required to produce these proteins from their polyprotein precursor: a viral protease, which functions in the cytosol to release the capsid protein; signalase, which makes two cleavages to separate the glycoproteins; and a protease of the Golgi complex that cleaves after double basic residues, to process the precursor form of one of the glycoproteins.

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Additional Information:Copyright © 1981 by the National Academy of Sciences Communicated by Ray D. Owen, December 11, 1980 We would like to thank E. Lenches for preparing the chicken fibroblasts; T.E. Crowley for help-with the 26S RNA isolation; H.V. Huang, T. Hunkapiller, and M. and R. Douglas for writing the computer programs and preparing the figures; J.R. Bell, M. W. Hunkapiller, and E.G. Strauss for helpful discussions; and L. Hood and T. Maniatis for their generosity in lending us supplies and equipment. We are especially grateful to H. Garoff, W.J. Welch, and B.M. Sefton for sending us preprints of their work. This work was supported by Grant PCM 77-26728 from the National Science Foundation, Grants GM 06965 and AI 10793 from the National Institutes of Health, and Biomedical Research Support Grant 507 RR 07003 from the National Institutes of Health. C.M.R. was supported by Training Grant GM 00086 from the National Institutes of Health. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
NSFPCM 77-26728
NIHGM 06965
NIHAI 10793
NIH507 RR 07003
NIHGM 00086
Subject Keywords:membrane glycoproteins; capsid protein; alphavirus; togavirus; protein processing
Issue or Number:4
PubMed Central ID:PMC319283
Record Number:CaltechAUTHORS:RICpnas81
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6250
Deposited By: Archive Administrator
Deposited On:29 Nov 2006
Last Modified:02 Oct 2019 23:30

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