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Reversible inhibition of interferon synthesis by puromycin: evidence for an interferon-specific messenger RNA

Wagner, Robert R. and Huang, Alice S. (1965) Reversible inhibition of interferon synthesis by puromycin: evidence for an interferon-specific messenger RNA. Proceedings of the National Academy of Sciences of the United States of America, 54 (4). pp. 1112-1118. ISSN 0027-8424. PMCID PMC219807.

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The capacity to synthesize interferon appears to be a latent cellular function which is induced by viral infection or other stimuli. The sequence of events can be conveniently studied in suspended cultures of Krebs-2 mouse ascites cells infected with Newcastle disease virus (NDV). Interferon synthesis in this model system begins 3-4 hr after infection and reaches maximal levels within 20 hr after viral infection. Previous studies have shown that interferon formation requires unimpaired cellular RNA synthesis during the first 4 hr after viral induction. Following this time, interferon continues to be formed even if RNA synthesis is inhibited by actinomycin. The present experiments provide further support for the hypothesis that a virus can switch on the cellular interferon gene by inducing the transcription of a messenger RNA that specifies the synthesis of the interferon protein. These two events in the cycle of interferon production can be dissected by suppression of protein synthesis with puromycin and of RNA synthesis with actinomycin.

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Additional Information:Copyright © 1965 National Academy of Sciences. Communicated by W. Barry Wood, Jr., August 24, 1965. We are grateful to Miss Ruth M. Snyder for invaluable assistance. This research was supported by grants from the National Science Foundation (GB 2576) and the National Institutes of Health (CA-02813).
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NSFGB 2576
Issue or Number:4
PubMed Central ID:PMC219807
Record Number:CaltechAUTHORS:20151203-135350370
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:62583
Deposited By: Donna Wrublewski
Deposited On:09 Dec 2015 00:25
Last Modified:03 Oct 2019 09:20

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