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Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi

Bagert, John D. and van Kessel, Julia C. and Sweredoski, Michael J. and Feng, Lihui and Hess, Sonja and Bassler, Bonnie L. and Tirrell, David A. (2016) Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi. Chemical Science, 7 (3). pp. 1797-1806. ISSN 2041-6520 . PMCID PMC4763989. https://resolver.caltech.edu/CaltechAUTHORS:20151207-090454052

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Abstract

Bacteria use a process of chemical communication called quorum sensing to assess their population density and to change their behavior in response to fluctuations in the cell number and species composition of the community. In this work, we identified the quorum-sensing-regulated proteome in the model organism Vibrio harveyi by bio-orthogonal non-canonical amino acid tagging (BONCAT). BONCAT enables measurement of proteome dynamics with temporal resolution on the order of minutes. We deployed BONCAT to characterize the time-dependent transition of V. harveyi from individual- to group-behaviors. We identified 176 quorum-sensing-regulated proteins at early, intermediate, and late stages of the transition, and we mapped the temporal changes in quorum-sensing proteins controlled by both transcriptional and post-transcriptional mechanisms. Analysis of the identified proteins revealed 86 known and 90 new quorum-sensing-regulated proteins with diverse functions, including transcription factors, chemotaxis proteins, transport proteins, and proteins involved in iron homeostasis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1039/c5sc03340cDOIArticle
http://pubs.rsc.org/en/Content/ArticleLanding/2016/SC/C5SC03340CPublisherArticle
http://www.rsc.org/suppdata/c5/sc/c5sc03340c/c5sc03340c1.pdfPublisherSupplementary Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763989/PubMed CentralArticle
ORCID:
AuthorORCID
Sweredoski, Michael J.0000-0003-0878-3831
Hess, Sonja0000-0002-5904-9816
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 2015 The Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Received 4th September 2015; Accepted 17th November 2015; First published online 23 Nov 2015. We thank Annie Moradian and Roxana Eggleston-Rangel for technical support with LC-MS/MS experiments. This work was supported by the Howard Hughes Medical Institute, National Institutes of Health (NIH) grant 5R01GM065859 and National Science Foundation (NSF) grant MCB-0343821 to BLB, and by NIH Grant R01GM062523 and the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office to DAT. SH and MJS were supported by the Gordon and Betty Moore Foundation, through Grant GBMF775, and the Beckman Institute. The content of the paper does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred. This article is part of themed collection: ISACS16: Challenges in Chemical Biology.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIH5R01GM065859
NSFMCB-0343821
NIHR01GM062523
Army Research Office (ARO)W911NF-09-0001
Gordon and Betty Moore FoundationGBMF775
Caltech Beckman InstituteUNSPECIFIED
Issue or Number:3
PubMed Central ID:PMC4763989
Record Number:CaltechAUTHORS:20151207-090454052
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151207-090454052
Official Citation:Time-resolved proteomic analysis of quorum sensing in Vibrio harveyi John D. Bagert, Julia C. van Kessel, Michael J. Sweredoski, Lihui Feng, Sonja Hess, Bonnie L. Bassler and David A. Tirrell Chem. Sci., 2016, 7, 1797-1806 DOI: 10.1039/C5SC03340C
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:62633
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Dec 2015 22:01
Last Modified:03 Oct 2019 09:20

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