CaltechAUTHORS
  A Caltech Library Service

The Biology of Isolated Chromatin

Bonner, James and Dahmus, Michael E. and Fambrough, Douglas and Huang, Ru-Chih C. and Marushige, Keiji and Tuan, Dorothy Y. H. (1968) The Biology of Isolated Chromatin. Science, 159 (3810). pp. 47-56. ISSN 0036-8075. doi:10.1126/science.159.3810.47. https://resolver.caltech.edu/CaltechAUTHORS:20151214-085353355

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20151214-085353355

Abstract

The isolated chromatin of higher organisms possesses several properties characteristic of the same chromatin in life. These include the presence of histone bound to DNA, the state of repression of the genetic material, and the ability to serve as template for the readout of the derepressed portion of the genome by RNA polymerase. The important respect in which isolated chromatin differs from the material in vivo, fragmentation of DNA into pieces shorter (5 x 10^6 to 20 x 10^6 molecular weight) than the original, does not appear to importantly alter such transcription. The study of isolated chromatin has already revealed the material basis of the restriction of template activity; it is the formation of a complex between histone and DNA. Chromatin isolated by the methods now available, together with the basis provided by our present knowledge of chromatin biochemistry and biophysics, should make possible and indeed assure rapid increase in our knowledge of chromosomal structure and of all aspects of the control of gene activity and hence of developmental processes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.159.3810.47 DOIArticle
http://www.sciencemag.org/content/159/3810/47PublisherArticle
http://www.jstor.org/stable/1723413JSTORArticle
Additional Information:© 1968 American Association for the Advancement of Science. The work reported in this article was supported by PHS grants GM-13762 and predecessor grants AM-03102, GM-03977, GM-05143; NSF grants G-25150; Herman Frasch Foundation; PHS training grant GM-0086 (Dahmus); NSF predoctoral fellowship (Fambrough); and the Arthur Mccallum Fund of the California Institute of Technology (Tuan).
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM-13762
U.S. Public Health Service (USPHS)AM-03102
U.S. Public Health Service (USPHS)GM-03977
U.S. Public Health Service (USPHS)GM-05143
NSFG-25150
Frasch FoundationUNSPECIFIED
U.S. Public Health Service (USPHS)GM-0086
NSF Predoctoral FellowshipUNSPECIFIED
CaltechUNSPECIFIED
Issue or Number:3810
DOI:10.1126/science.159.3810.47
Record Number:CaltechAUTHORS:20151214-085353355
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151214-085353355
Official Citation:The Biology of Isolated Chromatin James Bonner, Michael E. Dahmus, Douglas Fambrough, Ru-chih C. Huang, Keiji Marushige, and Dorothy Y. H. Tuan Science 5 January 1968: 159 (3810), 47-56. [DOI:10.1126/science.159.3810.47]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:62843
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Dec 2015 17:18
Last Modified:10 Nov 2021 23:07

Repository Staff Only: item control page