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The Biology of Isolated Chromatin

Bonner, James and Dahmus, Michael E. and Fambrough, Douglas and Huang, Ru-Chih C. and Marushige, Keiji and Tuan, Dorothy Y. H. (1968) The Biology of Isolated Chromatin. Science, 159 (3810). pp. 47-56. ISSN 0036-8075. doi:10.1126/science.159.3810.47.

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The isolated chromatin of higher organisms possesses several properties characteristic of the same chromatin in life. These include the presence of histone bound to DNA, the state of repression of the genetic material, and the ability to serve as template for the readout of the derepressed portion of the genome by RNA polymerase. The important respect in which isolated chromatin differs from the material in vivo, fragmentation of DNA into pieces shorter (5 x 10^6 to 20 x 10^6 molecular weight) than the original, does not appear to importantly alter such transcription. The study of isolated chromatin has already revealed the material basis of the restriction of template activity; it is the formation of a complex between histone and DNA. Chromatin isolated by the methods now available, together with the basis provided by our present knowledge of chromatin biochemistry and biophysics, should make possible and indeed assure rapid increase in our knowledge of chromosomal structure and of all aspects of the control of gene activity and hence of developmental processes.

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Additional Information:© 1968 American Association for the Advancement of Science. The work reported in this article was supported by PHS grants GM-13762 and predecessor grants AM-03102, GM-03977, GM-05143; NSF grants G-25150; Herman Frasch Foundation; PHS training grant GM-0086 (Dahmus); NSF predoctoral fellowship (Fambrough); and the Arthur Mccallum Fund of the California Institute of Technology (Tuan).
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM-13762
U.S. Public Health Service (USPHS)AM-03102
U.S. Public Health Service (USPHS)GM-03977
U.S. Public Health Service (USPHS)GM-05143
Frasch FoundationUNSPECIFIED
U.S. Public Health Service (USPHS)GM-0086
NSF Predoctoral FellowshipUNSPECIFIED
Issue or Number:3810
Record Number:CaltechAUTHORS:20151214-085353355
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Official Citation:The Biology of Isolated Chromatin James Bonner, Michael E. Dahmus, Douglas Fambrough, Ru-chih C. Huang, Keiji Marushige, and Dorothy Y. H. Tuan Science 5 January 1968: 159 (3810), 47-56. [DOI:10.1126/science.159.3810.47]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:62843
Deposited By: Tony Diaz
Deposited On:14 Dec 2015 17:18
Last Modified:10 Nov 2021 23:07

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