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The production of nitric oxide in endothelial cells by amphiphiles

Conforto, A. and Dudek, R. and Hoffmann, M. R. and Bing, R. J. (1994) The production of nitric oxide in endothelial cells by amphiphiles. Life Sciences, 54 (16). pp. 1143-1153. ISSN 0024-3205.

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Lysophosphatidylcholine, an endogenous detergent is an endothelium-dependent smooth muscle relaxant, which acts through the release of nitric oxide. It is known to activate a number of membrane-bound enzymes. Because of the relationship between detergent action, relaxation of endothelium-intact rabbit aortic strips and the release of nitric oxide, we considered the possibility that other amphiphiles also produce nitric oxide from endothelial cells. We therefore investigated the effect of digitonin on relaxation of precontracted rabbit aortic strips and the release of nitric oxide from freshly harvested bovine endothelial cells as determined by chemiluminescence. We found that both digitonin and LPC release nitric oxide and that this process is inhibited by the NO synthase inhibitor Nω-Nitro-L-Arginine Methyl Ester (L-NNAME).

Item Type:Article
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Hoffmann, M. R.0000-0001-6495-1946
Additional Information:© 1994 Elsevier Science Ltd. Received in final form January 25, 1994. The original work for this study was supported by grants from the Council for Tobacco Research, USA, Inc., New York and The Margaret W. and Herbert Hoover, Jr. Foundation, Pasadena, California. Dr. Conforto is the recipient of the Boswell Fellowship, jointly administered by the Huntington Medical Research Institutes and the California Institute of Technology. The authors would like to thank Matthew Vanasco and Alice Alegria for technical assistance and Linda Dechaine for secretarial help.
Funding AgencyGrant Number
Council for Tobacco ResearchUNSPECIFIED
Margaret W. and Herbert Hoover, Jr. FoundationUNSPECIFIED
Huntington Medical Research Institute (HMRI)UNSPECIFIED
Subject Keywords:endothelial cells; nitric oxide; amphiphiles; digitonin; lysophosphatidylcholine
Issue or Number:16
Record Number:CaltechAUTHORS:20151214-155200160
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Official Citation:A. Conforto, R. Dudek, M.R. Hoffmann, R.J. Bing, The production of nitric oxide in endothelial cells by amphiphiles, Life Sciences, Volume 54, Issue 16, 1994, Pages 1143-1153, ISSN 0024-3205, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:62905
Deposited By: Tony Diaz
Deposited On:15 Dec 2015 00:08
Last Modified:03 Mar 2020 13:01

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