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Real-Time Lineage Analysis to Study Cell Division Orientation in the Arabidopsis Shoot Meristem

Tobin, Cory and Meyerowitz, Elliot M. (2016) Real-Time Lineage Analysis to Study Cell Division Orientation in the Arabidopsis Shoot Meristem. In: Plant Cell Division: Methods and Protocols. Methods in Molecular Biology. No.1370. Springer , New York, NY, pp. 147-167. ISBN 978-1-4939-3141-5. https://resolver.caltech.edu/CaltechAUTHORS:20151216-110613825

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Abstract

Cells in the Arabidopsis shoot apical meristem are small and divide frequently throughout the life-time of the organism making them good candidates for studying the mechanisms of cell division in plants. But tracking these cell divisions requires multiple images to be taken of the same specimen over time which means the specimen must stay alive throughout the process. This chapter provides details on how to prepare plants for live imaging, keep them alive and growing through multiple time points, and how to process the data to extract cell boundary coordinates from three-dimensional images.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/978-1-4939-3142-2_12DOIArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2015 Springer Science+Business Media New York.
Subject Keywords:Live imaging; Image processing; Cell division; Segmentation; Arabidopsis
Series Name:Methods in Molecular Biology
Issue or Number:1370
DOI:10.1007/978-1-4939-3142-2_12
Record Number:CaltechAUTHORS:20151216-110613825
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151216-110613825
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:62978
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Dec 2015 19:49
Last Modified:10 Nov 2021 23:09

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