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The piRNA Pathway Guards the Germline Genome Against Transposable Elements

Fejes Tóth, Katalin and Pezic, Dubravka and Stuwe, Evelyn and Webster, Alexandre (2016) The piRNA Pathway Guards the Germline Genome Against Transposable Elements. In: Non-coding RNA and the Reproductive System. Advances in Experimental Medicine and Biology. No.886. Springer , Dordrecht, Netherlands, pp. 51-77. ISBN 978-94-017-7415-4. PMCID PMC4991928. https://resolver.caltech.edu/CaltechAUTHORS:20151216-111221025

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Abstract

Transposable elements (TEs) have the capacity to replicate and insert into new genomic locations. This contributs significantly to evolution of genomes, but can also result in DNA breaks and illegitimate recombination, and therefore poses a significant threat to genomic integrity. Excess damage to the germ cell genome results in sterility. A specific RNA silencing pathway, termed the piRNA pathway operates in germ cells of animals to control TE activity. At the core of the piRNA pathway is a ribonucleoprotein complex consisting of a small RNA, called piRNA, and a protein from the PIWI subfamily of Argonaute nucleases. The piRNA pathway relies on the specificity provided by the piRNA sequence to recognize complementary TE targets, while effector functions are provided by the PIWI protein. PIWI-piRNA complexes silence TEs both at the transcriptional level – by attracting repressive chromatin modifications to genomic targets – and at the posttranscriptional level – by cleaving TE transcripts in the cytoplasm. Impairment of the piRNA pathway leads to overexpression of TEs, significantly compromised genome structure and, invariably, germ cell death and sterility. The piRNA pathway is best understood in the fruit fly, Drosophila melanogaster, and in mouse. This Chapter gives an overview of current knowledge on piRNA biogenesis, and mechanistic details of both transcriptional and posttranscriptional TE silencing by the piRNA pathway. It further focuses on the importance of post-translational modifications and subcellular localization of the piRNA machinery. Finally, it provides a brief description of analogous pathways in other systems.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/978-94-017-7417-8_4DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4991928/PubMed CentralArticle
ORCID:
AuthorORCID
Fejes Tóth, Katalin0000-0001-6558-2636
Additional Information:© 2015 Springer Science+Business Media Dordrecht.
Subject Keywords:piRNA – Small RNA – Argonautes – Piwi proteins – TE – Transposon – Transposable element – Tudor domain – ranscriptional silencing – Posttranscriptional silencing –Heterochromatin – H3K9me3 – DNA methylation – Ping-Pong cycle – Germ granules – Nuage – Pole plasm – Pi-bodies –Intramitochondrial cement
Series Name:Advances in Experimental Medicine and Biology
Issue or Number:886
PubMed Central ID:PMC4991928
Record Number:CaltechAUTHORS:20151216-111221025
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20151216-111221025
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:62979
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Dec 2015 21:10
Last Modified:23 Apr 2020 22:33

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